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T6522
Trypsin inhibitor from Glycine max (soybean)
powder, BioReagent, suitable for cell culture
Trypsin inhibitor from Glycine max (soybean)
Synonim(y):
SBTI
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
25 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 289,00 zł |
100 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 580,00 zł |
250 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1240,00 zł |
500 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2520,00 zł |
1 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2680,00 zł |
5 x 100 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3270,00 zł |
5 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 11 680,00 zł |
Informacje o tej pozycji
Specific activity:
(One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.)
Biological source:
Glycine max (soybean)
Concentration:
10 mg/mL
289,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
Glycine max (soybean)
Quality Segment
type
Type I-S
product line
BioReagent
form
powder
specific activity
(One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.)
mol wt
20.1 kDa
concentration
10 mg/mL
technique(s)
cell culture | mammalian: suitable
solubility
balanced salt solution: 1 mg/mL, serum-free medium: soluble
shipped in
ambient
storage temp.
2-8°C
Application
This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Analysis Note
One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.
Other Notes
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Phaseolus limensis (lima bean) | biological source - |
| specific activity (One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.) | specific activity - | specific activity - | specific activity ≥7,000 units/mg protein |
| concentration 10 mg/mL | concentration - | concentration - | concentration - |
| form powder | form lyophilized powder | form crude powder | form lyophilized solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
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Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
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flash_point_c
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What is the Department of Transportation shipping information for this product?
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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