SAE0046

Carboxypeptidase A from bovine pancreas

(Type II-PMSF treated), ≥20 units/mg protein

• Synonim(y): Carboxypeptidase A, Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
• Numer CAS: 11075-17-5
• Numer EC enzymu: 3.4.17.1 (BRENDA, IUBMB)
• Numer MDL: MFCD00130718
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bovine pancreas
• Poziom jakości: 200
• Postać: aqueous suspension
• jakość: (Type II-PMSF treated)
• aktywność właściwa: ≥20 units/mg protein
• masa cząsteczkowa: ~35 kDa
• oczyszczone przez: 2× crystallization
• zanieczyszczenia: ≤0.05 BTEE units/mg protein chymotrpsin; ≤10 BAEE units/mg protein trypsin
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Carboxypeptidase A (CPA) is a secreted protease is liberated after the activation of mast cells to facilitate acute anaphylaxis. Carboxypeptidase A has a long half-life in vivo, when compared to other secreted proteases.

Zastosowanie

Carboxypeptidase A from bovine pancreas has been used in in vitro simulated digestion.

Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.

Działania biochem./fizjol.

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.†

Definicja jednostki

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Uwaga dotycząca przygotowania

Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.

Komentarz do analizy

Protein determined by E1%/278

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable