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R5500

Sigma-Aldrich

Ribonuclease A from bovine pancreas

Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

Synonim(y):

Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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About This Item

Numer CAS:
9001-99-4
Numer EC enzymu:
3.1.27.5 (BRENDA, IUBMB)
Numer WE:
232-646-6
Numer MDL:
MFCD00132181
Kod UNSPSC:
12352204
NACRES:
NA.54

pochodzenie biologiczne

bovine pancreas

Poziom jakości

300

typ

Type XII-A

Próba

≥90% (SDS-PAGE)

Postać

lyophilized powder

aktywność właściwa

75-125 Kunitz units/mg protein

masa cząsteczkowa

~13,700

metody

cell based assay: suitable

zanieczyszczenia

salt, essentially free

przydatność

suitable for mRNA or total RNA extracted from cells and tissues

Zastosowanie

diagnostic assay manufacturing

obecność zanieczyszczeń

protease, essentially free

temp. przechowywania

−20°C

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

Klucz InChI

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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Opis ogólny

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Zastosowanie

  • RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
  • RNase A is also used in RNA sequence analysis and protection assays.
  • RNase A has been used as a tool for computer-aided drug design.
  • RNase A supports the analysis of RNA sequences.
  • RNase A hydrolyze RNA contained in protein samples.
  • Purification of DNA is supported by RNase A.

Działania biochem./fizjol.

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Cechy i korzyści

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Uwaga dotycząca przygotowania

Salt fractionated and chromatographically purified.

Komentarz do analizy

Protein determined by E.
This page may contain text that has been machine translated.

Zastosowanie

Numer produktu
Opis
Cennik

inhibitor

Numer produktu
Opis
Cennik

Piktogramy

Health hazard
GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H334

Zwroty wskazujące środki ostrożności

P261 - P284 - P501

Klasyfikacja zagrożeń

Resp. Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Vlad Zabrouskov et al.
Biochemistry, 45(3), 987-992 (2006-01-18)
Although deamidation at asparagine and glutamine has been found in numerous studies of a variety of proteins, in almost all cases the analytical methodology that was used could detect only a single site of deamidation. For the extensively studied case
Amy B Emerman et al.
Methods in molecular biology (Clifton, N.J.), 1413, 303-324 (2016-05-20)
RNAs associate with the mitotic spindle in a variety of organisms, where they can spatially regulate protein production, ensure their proper segregation during cell division, or perform translation-independent roles in spindle formation. The identification of spindle-associated RNAs is an important
Amaya Albalat et al.
Methods in molecular biology (Clifton, N.J.), 984, 153-165 (2013-02-07)
The analysis of proteins and peptides in biological fluids is becoming more important as they are potential sources of diagnostic biomarkers of disease. The complexity of body fluids is such that no single technique can both identify and quantify all
Xin-Miao Fu et al.
Biochimica et biophysica acta, 1814(4), 487-495 (2011-01-18)
Protein disulfide isomerase (PDI) and its pancreatic homolog (PDIp) are folding catalysts for the formation, reduction, and/or isomerization of disulfide bonds in substrate proteins. However, the question as to whether PDI and PDIp can directly attack the native disulfide bonds
Romina Ponzielli et al.
Nucleic acids research, 36(21), e144-e144 (2008-10-23)
High-throughput, microarray-based chromatin immunoprecipitation (ChIP-chip) technology allows in vivo elucidation of transcriptional networks. However this complex is not yet readily accessible, in part because its many parameters have not been systematically evaluated and optimized. We address this gap by systematically
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Protokoły

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

Test enzymatyczny rybonukleazy A (EC 3.1.27.5)

Procedura ta może być stosowana do oznaczania aktywności rybonukleazy A (RNazy A).

Chromatograms

application for HPLC

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