R5125

Ribonuclease A from bovine pancreas

Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

• Synonim(y): Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
• Numer CAS: 9001-99-4
• Numer EC enzymu: 3.1.27.5 (BRENDA, IUBMB)
• Numer WE: 232-646-6
• Numer MDL: MFCD00132181
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bovine pancreas
• Poziom jakości: 300
• typ: Type III-A
• Próba: ≥85% RNase A basis (SDS-PAGE)
• Postać: lyophilized powder
• aktywność właściwa: 85-140 Kunitz units/mg protein
• masa cząsteczkowa: ~13,700
• metody: cell based assay: suitable
• zanieczyszczenia: salt, essentially free
• przydatność: suitable for molecular biology
• Zastosowanie: diagnostic assay manufacturing
• obecność zanieczyszczeń: protease, essentially free
• temp. przechowywania: −20°C
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• Klucz InChI: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Opis

Opis ogólny

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Zastosowanie

• RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
• RNase A is also used in RNA sequence analysis and protection assays.
• RNase A has been used as a tool for computer-aided drug design.
• RNase A supports the analysis of RNA sequences.
• RNase A hydrolyze RNA contained in protein samples.
• Purification of DNA is supported by RNase A.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess nickel-dependent oxidative cross-linking of a protein. Ribonuclease A from bovine pancreas has also been used in a study to investigate equilibrium constants for nonspecific binding of proteins to DNA.

Działania biochem./fizjol.

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Cechy i korzyści

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Uwaga dotycząca przygotowania

Salt fractionated and chromatographically purified.

Komentarz do analizy

Protein determined by E.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)