IP04
Protein G Plus-Agarose Suspension
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
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About This Item
Kod UNSPSC:
41116133
NACRES:
NA.56
Polecane produkty
Postać
slurry (Liquid)
zawiera
≤0.1% sodium azide as preservative
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
do not freeze
metody
immunoprecipitation (IP): suitable
przydatność
suitable for immunoprecipitation
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Opis ogólny
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
Ostrzeżenie
Toxicity: Standard Handling (A)
Inne uwagi
Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Powiązane treści
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
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