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N1127

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

Synonym(s):

ONPG , o-Nitrophenyl β-D-galactopyranoside, ONPG

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250 mg

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$37.90
500 mg

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$52.60
1 g

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$57.80
5 g

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$201.00
25 g

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$799.00

About This Item

Empirical Formula (Hill Notation):
C12H15NO8
CAS Number:
369-07-3
Molecular Weight:
301.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24897461
EC Number:
206-716-1
Beilstein/REAXYS Number:
92207
MDL number:
MFCD00063255

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grade

Molecular Biology

Quality Segment

200

assay

≥98% (enzymatic)

form

powder

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1

InChI key

KUWPCJHYPSUOFW-YBXAARCKSA-N

General description

Chromogenic substrate for β-galactosidase
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Application

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Preparation Note

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

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2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic)

Sigma-Aldrich

N1127

2-Nitrophenyl β-D-galactopyranoside

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Sigma-Aldrich

N9003

4-Nitrophenyl N-acetyl-β-D-galactosaminide

4-Nitrophenyl β-D-galacto­pyran­oside ≥98% (enzymatic)

Sigma-Aldrich

N1252

4-Nitrophenyl β-D-galacto­pyran­oside

2-Nitrophenyl β-D-glucopyranoside powder

Sigma-Aldrich

N8016

2-Nitrophenyl β-D-glucopyranoside

assay

≥98% (enzymatic)

assay

≥98%

assay

≥98% (TLC), ≥98% (enzymatic)

assay

≥99% (TLC)

grade

Molecular Biology

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

11 - Combustible Solids

wgk

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flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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