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M7027

Sigma-Aldrich

Methyl cellulose

viscosity: 15 cP, BioReagent, suitable for cell culture

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100 G
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667,00 zł

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100 G
667,00 zł
250 G
1500,00 zł

About This Item

Numer CAS:
9004-67-5
Numer MDL:
MFCD00081763
Kod UNSPSC:
12352201
Identyfikator substancji w PubChem:
24897135
NACRES:
NA.75

667,00 zł

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pochodzenie biologiczne

synthetic (organic)

linia produktu

BioReagent

Formularz

powder

metody

cell culture | mammalian: suitable

lepkość

12-18 cP, 2 % in H2O(20 °C)

rozpuszczalność

water: 50 mg/mL, clear to hazy

ciąg SMILES

[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]

Klucz InChI

YLGXILFCIXHCMC-JHGZEJCSSA-N

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Opis ogólny

Methyl cellulose is obtained by etherification of cellulose. This commercial cellulose ether exhibits amphiphilic properties and original physicochemical properties. Methyl cellulose is enzyme resistant, non-digestible, non-allergic, and non-toxic.

Zastosowanie

Methyl cellulose has been used:
  • as a vehicle for metformin drug during oral gavage
  • as a component of mammary epithelial cell growth medium for mammosphere culture
  • as a supplement in spheroid 3D co-cultures for spheroid invasion assays using hanging drop protocol

Methyl cellulose is used to create semi-solid matrices,[1][2] as a cell culture gel, for culture of colony forming cells[3][4] such as CFU-G; CFU-GM; cells and other cells used in toxicity assays. Low viscosity, not for plaquing assays or cloning.

Działania biochem./fizjol.

Methyl cellulose finds applications in various industries such as in the food industry it is used as an emulsifier, texturing agent, thickener, and gelling additive. Methyl cellulose is also used in various cosmetics and personal care products such as hair shampoos, hair styling products, liquid soaps, body washes, lotions and creams, and toothpaste to yield their characteristic thick consistency. In addition, it serves as an additive for pharmaceuticals and is used in the production of capsules for nutritional supplements. Methyl cellulose is used as an alternative to glycerol in a cryopreservation medium. It is used along with culture supplements to enhance proliferation and differentiation in multiple or single lineage-committed hematopoietic progenitor cells. This cellulose derivative also has the potential to prevent the loss of adhesive properties and cell functions of various cell types including human skin fibroblasts, human melanoma cells, and mouse lung fibroblasts cultured in suspension.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000434092
0000434092
0000409636
0000395386
0000395387

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Barley beta-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts
Fuste NP, et al.
Carbohydrate Polymers, 389-398 (2019)
Chandler Warr et al.
ACS applied bio materials, 3(4), 2239-2244 (2020-05-30)
We report a non-cytotoxic resin compatible with and designed for use in custom high-resolution 3D printers that follow the design approach described in Gong et al., Lab Chip 17, 2899 (2017). The non-cytotoxic resin is based on a poly(ethylene glycol)
High throughput and automatic colony formation assay based on impedance measurement technique
Lei KF, et al.
Analytical and Bioanalytical Chemistry, 409(12), 3271-3277 (2017)
Large variability in the motility of spiroplasmas in media of different viscosities
Boudet JF, et al.
Scientific Reports, 8(1), 17138-17138 (2018)
Dissociated effect and Chemosensitive enhancement of tumor spheroids influenced by an electric field in a microdevice
Huang Chun-Hao, et al.
Biomedical Microdevices, 20(3), 70-70 (2018)
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Protokoły

Metody hodowli hematopoetycznych komórek macierzystych

Protokoły hodowli hematopoetycznych komórek macierzystych do izolacji, ekspansji i różnicowania komórek CD34+, w tym testy CFU.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Questions

1–10 of 13 Questions  

  1. How to practically reach a 1% solution of Methylcellulose in cell culture media that can't be heated excessively?

    1 answer

    1. This product is supplied in powder form. The solubility in water is 50 mg/mL, resulting in a hazy solution. To prepare a 1% Methylcellulose solution in cell culture media from this stock solution, follow these steps:
      A 1% solution requires 1 g of solute per 100 mL of solution, equivalent to 10 mg/mL. The stock solution is 50 mg/mL. To achieve this, take 20 mL of the 50 mg/mL stock solution and dilute it to a total volume of 100 mL.

      Helpful?

  2. I would like to use methylcellulose in my cell culture media to optimize organoids in suspension. What methylcellulose product would be the most appropriate? And can I dissolve it directly into the cold media?

    1 answer

    1. The methylcellulose products available are not currently tested for use in organoid suspension. However, a previously available product, S3812, was an IMDM-based stem cell culture medium that included a methylcellulose component with a 4000 cps viscosity, at a concentration of 1%. This would correspond to product M0512. The solubility in cold medium has not been determined. Please see the link below to review the general methylcellulose datasheet which includes a viscosity comparison table:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/183/010/m6385pis.pdf

      Helpful?

  3. Can the M7027 pass through a 0.2um filter?

    1 answer

    1. Methyl cellulose has a high molecular weight, and the viscosity of methyl cellulose solutions can also impede its ability to pass through such fine filters, as it can clog the filter pores. For sterilization, a 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of solution.

      Helpful?

  4. Does this product need to be sterilized after dissolving for use in cell culture?

    1 answer

    1. Solutions of this material require sterilization for cell culture applications. A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of the solution.

      Helpful?

  5. Hello sir, I need to know molar mass or molecular weight of this code of Methyl cellulose or tell me how can i calculate can you help me?

    1 answer

    1. The molecular weight is not analyzed on a lot to lot basis. The approximate molecular weight is 88,000 Da with methoxy substitution between 27.5 - 31.5% (weight).

      Helpful?

  6. Can I use this to make 0.5% solution in water which will be used as the solvent to dissolve neratinib and administrate to mice through oral gavage?

    1 answer

    1. This product can be solubilized in water at 50mg/ml which is a 0.5% solution. This product is tested for use in cell cultue, however it has not been tested for in vivo applications. Suitability for in vivo use will need to be validated by the end-user. See the link below for a general product information sheet for Methyl Cellulose. Note product M7140, which is the general research grade of this product. All details for M7140 will apply to M7027.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/785/m0512pis.pdf

      Helpful?

  7. Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?

    1 answer

    1. Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media.  Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.

      Helpful?

  8. How do I store a stock solution of Methyl cellulose, Product M7027?

    1 answer

    1. A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How can I make a solution of Methyl cellulose, Product M7027?

    1 answer

    1. There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols.

      Helpful?

1–10 of 13 Questions  

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