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Merck

L2769

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder

Synonim(y):

Phaseolus vulgaris agglutinin, PHA

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2 MG
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5 MG
986,00 zł
10 MG
1810,00 zł

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2 MG
860,00 zł
5 MG
986,00 zł
10 MG
1810,00 zł

About This Item

Kod UNSPSC:
12352202
NACRES:
NA.32

860,00 zł


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pochodzenie biologiczne

Phaseolus vulgaris

Formularz

lyophilized powder

siła działania

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.)
≤1.0 μg/mL leucoagglutination activity
≤5 μg/mL mitogenic activity

skład

Protein, ≥60% biuret

zanieczyszczenia

salt, free

temp. przechowywania

2-8°C

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Opis ogólny

Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa.[1]

Zastosowanie

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • as a positive control in ELISPOT (enzyme-linked immunospot) assay[2]
  • to induce Th2 polarization for T-cell differentiation in vitro[3]
  • as controls in the lymphocyte proliferation assays[4]

Działania biochem./fizjol.

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Uwaga dotycząca przygotowania

Purified by a modification of the method of Leavitt.[5]

Komentarz do analizy

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Odwiedź Bibliotekę dokumentów

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Rakotosamimanana N, et al.
Clinical and Vaccine Immunology : CVI, 17(7), 1094-1103 (2010)
Linc-MAF-4 regulates Th1/Th2 differentiation and is associated with the pathogenesis of multiple sclerosis by targeting MAF
Zhang F, et al.
Faseb Journal, 31(2), 519-525 (2016)
Fan Yang et al.
Gene therapy, 29(1-2), 41-54 (2021-01-13)
Adeno-Associated Virus (AAV)-based gene therapy vectors are in development for many inherited human disorders. In nonclinical studies, cellular immune responses mediated by cytotoxic T cells may target vector-transduced cells, which could impact safety and efficacy. Here, we describe the bioanalytical
Safety and immunogenicity of the Plasmodium falciparum merozoite surface protein-3 long synthethic peptide (MSP3-LSP) malaria vaccine in healthy, semi-immune adult males in Burkina Faso, West Africa
Sirima SB, et al.
Vaccine, 25(14), 2723-2732 (2007)
Kirsten E Lyke et al.
Vaccine, 27(15), 2171-2176 (2009-04-10)
The development of a safe and effective malaria vaccine is impeded by the complexity of the Plasmodium life cycle. A vaccine that elicits both cell-mediated and humoral immune responses might be needed for protection against this multistage parasitic infection. Apical

Produkty

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Questions

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer
    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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