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L2769

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder

Synonim(y):

Phaseolus vulgaris agglutinin, PHA

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Do Państwa/SKUDostępnośćCena netto
2 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
577,00 zł
5 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1030,00 zł
10 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1880,00 zł

Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Phaseolus vulgaris

577,00 zł

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biological source

Phaseolus vulgaris

Quality Segment

200

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity

composition

Protein, ≥60% biuret

impurities

salt, free

storage temp.

2-8°C

General description

Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • as a positive control in ELISPOT (enzyme-linked immunospot) assay
  • to induce Th2 polarization for T-cell differentiation in vitro
  • as controls in the lymphocyte proliferation assays

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Preparation Note

Purified by a modification of the method of Leavitt.[1]

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
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L8754L2646L1668
Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder

Sigma-Aldrich

L2769

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder

Sigma-Aldrich

L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-M, lyophilized powder

Sigma-Aldrich

L2646

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Sigma-Aldrich

L1668

Lectin from Phaseolus vulgaris (red kidney bean)

biological source

Phaseolus vulgaris

biological source

Phaseolus vulgaris

biological source

-

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

salt, free

impurities

salt, free

impurities

salt, free

impurities

-

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤5 μg/mL mitogenic activity, ≤1.0 μg/mL leucoagglutination activity

potency

≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity

potency

<40 μg per mL agglutination activity

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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