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L8754

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Synonim(y):

Phaseolus vulgaris agglutinin, PHA

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About This Item

Numer WE:
232-718-7
Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

Phaseolus vulgaris

Postać

lyophilized powder

siła działania

≤62.5 μg per mL agglutination activity
≤10 μg/mL mitogenic activity

zanieczyszczenia

salt, free

temp. przechowywania

2-8°C

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Opis ogólny

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

Zastosowanie

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

Działania biochem./fizjol.

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Komentarz do analizy

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Andrew Z Wang et al.
The Journal of experimental biology, 223(Pt 20) (2020-09-13)
Limited resources must be partitioned among traits that enhance fitness. Although survival-related traits often trade off with reproduction, survival-related traits themselves may trade off with each other under energy limitations. Whole-organism performance and the immune system both enhance survival, yet
Qianting Yang et al.
Thorax, 75(7), 576-583 (2020-03-24)
Biomarker-based tests for diagnosing TB currently rely on detecting Mycobacterium tuberculosis (Mtb) antigen-specific cellular responses. While this approach can detect Mtb infection, it is not efficient in diagnosing TB, especially for patients who lack aetiological evidence of the disease. We
Phytohemagglutinin content in fresh kidney bean in China
Sun Y, et al.
International journal of food properties, 22(1), 405-413 (2019)
A R S Badari Nath et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 134, 143-147 (2014-07-10)
Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco. This PHA (Gibco) was found to be very expensive, hence other inexpensive sources
Pheasant sexual ornaments reflect nutritional conditions during early growth
Ohlsson T, et al.
Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character. Royal Society (Great Britain), 269(1486), 21-27 (2002)
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Produkty

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

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