L2650
Lectin from Glycine max (soybean)
peroxidase conjugate, lyophilized powder
Synonim(y):
SBA, Soybean agglutinin
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
Glycine max (soybean)
białko sprzężone
peroxidase conjugate
Próba
85.00-100.00% (Warburg-Christian)
Formularz
lyophilized powder
siła działania
<80 μg per mL agglutination activity
peroxidase activity
25-100 units/mg protein
skład
Protein, ~95% modified Warburg-Christian
metody
western blot: suitable
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
soybean ... LE1(100818710)
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Zastosowanie
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Działania biochem./fizjol.
SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.
Definicja jednostki
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.
Postać fizyczna
Contains sodium citrate
Uwaga dotycząca przygotowania
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan,[1] which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Komentarz do analizy
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.
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Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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