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M1778

Mucin from porcine stomach

Type III, bound sialic acid 0.5-1.5 %, partially purified powder

Synonim(y):

MUC

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Do Państwa/SKUDostępnośćCena netto
10 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
762,00 zł
100 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2540,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352202
EC Number:
282-010-7
NACRES:
NA.61
MDL number:
Form:
partially purified powder
Biological source:
Porcine stomach

762,00 zł


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biological source

Porcine stomach

Quality Segment

type

Type III

form

partially purified powder

composition

bound sialic acid, 0.5-1.5%

technique(s)

microbiological culture: suitable

solubility

NaOH: soluble 20 mg/mL

storage temp.

2-8°C

General description

Mucins or mucus glycoproteins are the main macromolecular components of mammalian mucus. It was also used in a study to evaluate the sputum smears concentrated by cyto-centrifugation for detection of acid-fast bacilli.

Application

Mucin from porcine stomach was used in studies on the binding site of the galactose-specific agglutinin PA-IL from Pseudomonas aeruginosa.

Biochem/physiol Actions

Mucus forms the protective cover for all epithelial surfaces. The major structural component of mucus is gel-forming mucins. The mucus layer covering the intestinal epithelium has the MUC2 mucin as its central molecule. MUC2 is produced by goblet cells.

Preparation Note

Prepared according to the method of Glenister, et al., for use in complex growth media for dental plaque bacteria.
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Ta pozycja
M3895M2378G2500
description

Type III, bound sialic acid 0.5-1.5 %, partially purified powder

description

Type I-S

description

Type II

description

gel strength 300, Type A

technique(s)

microbiological culture: suitable

technique(s)

electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

Porcine stomach

biological source

bovine submaxillary glands

biological source

Porcine stomach

biological source

Porcine skin

form

partially purified powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

solubility

NaOH: soluble 20 mg/mL

solubility

-

solubility

1 M NaOH: soluble 20 mg/mL

solubility

H2O: soluble 50 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–7 of 7 Questions  
  1. Can I reconstitute the mucin so that it forms a saliva/snot-like substance? I can't seem to find any papers that have done this...

    1 answer
    1. Mucins are soluble in water, forming hazy viscous solutions.

      Helpful?

  2. What are the differences between Mucin from porcine stomach Products M2378 and M1778?

    1 answer
    1. Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website.

      Helpful?

  3. How can I solubilize Product M1778, Mucin from porcine stomach?

    1 answer
    1. Mucin is not readily soluble in aqueous solutions. Turbid "solutions" will form at about 5 mg/mL in 0.1 M acetate buffer, pH 5.0. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Is Product M1778, Mucin from porcine stomach, a purified preparation of mucin?

    1 answer
    1. This product is a partially purified preparation of mucin based on the method of Glenister cited on the product's webpage.

      Helpful?

  6. What does the "Type" designation in Mucin from porcine stomach mean?

    1 answer
    1. The type designation does not have any particular biological significance.  It is used to emphasize differences in the listings of this product.

      Helpful?

  7. What is the molecular weight of Product M1778, Mucin from porcine stomach?

    1 answer
    1. This product is partially purified from crude mucin, M2378.  Sigma has not determined the molecular weight of this product. Neither has the sugar and amino acid composition been determined.

      Helpful?

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