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Merck

GE28-9924-74

HiScreen Capto Blue

Cytiva 28-9924-74, pack of 1 column

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About This Item

Kod UNSPSC:
23151817
NACRES:
NA.56

opakowanie

pack of 1 column

producent / nazwa handlowa

Cytiva 28-9924-74

Parametry

1.2 ml/min - 2.3 ml/min flow rate
44 psi

wymiary złoża

7.7 mm × 100 mm

objętość złoża

4.7 mL

pojemność

~25 mg binding capacity(human serum albumin/ml medium)

temp. przechowywania

2-8°C

Opis ogólny

HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

Zastosowanie

HiScreen Capto Blue is prepacked with the base matrix Capto to which Cibacron Blue 3G is covalently bound via a hydrophilic spacer immobilized with a stable amine bond. Capto Blue is highly chemically stable and has a more rigid Agarose base matrix than Blue Sepharose 6 Fast Flow. This allows the use of faster flow rates and larger sample volumes, leading to higher throughput and improved process economy. The application area is the same as for Blue Sepharose Fast FLow (e.g., purification of albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons, and related Proteins). The main benefit of Capto Blue compared to Blue Sepharose 6 Fast FLow is its improved chemical stability provided with a new coupling chemistry involving a spacer arm. It tolerates repeated cleaning-in-place and sanitization at both high and Low pH as well as autoclaving. Also the leakage of Cibacron Blue is reduced as compared to Blue Sepharose 6 Fast Flow.

Cechy i korzyści

  • Prepacked with Capto Blue
  • Excellent for method optimization and parameter screening due to the 10 cm bed height
  • Easily connected in series to achieve 20 cm bed height
  • Small bed volume for fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Inne uwagi

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informacje prawne

Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
This page may contain text that has been machine translated.

Piktogramy

Flame

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Kod klasy składowania

3 - Flammable liquids


Certyfikaty analizy (CoA)

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Produkty

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protokoły

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.

Ta strona pokazuje, jak oczyszczać lub usuwać albuminy za pomocą Blue Sepharose i Capto Blue firmy Cytiva.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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