GE28-4017-45

GSTrap^™ 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

• Kod UNSPSC: 23151816
• NACRES: NA.56

Właściwości

• ligand: glutathione
• opakowanie: pack of 5 × 1 mL
• producent / nazwa handlowa: Cytiva 28-4017-45
• Parametry: <4 mL/min flow rate; 42 psi (H₂O at 25°C)
• wymiary złoża: 7 mm × 25 mm
• objętość złoża: 1 mL
• kolumna śr. wewn.: 7 mm
• macierz: 4% agarose
• wielkość cząstki: 45-165 μm
• średnia średnica: 90 μm
• cleaning: 4-13
• working range: 4-13
• pojemność: >25 mg binding capacity (horse liver GST/ml medium)

Opis

Opis ogólny

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Zastosowanie

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Cechy i korzyści

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Inne uwagi

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informacje prawne

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H226
• Zwroty wskazujące środki ostrożności: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Kod klasy składowania: 3 - Flammable liquids