F3040
Monoclonal ANTI-FLAG® M1 antibody produced in mouse
clone M1, purified immunoglobulin, buffered aqueous solution
Synonim(y):
Anti-ddddk, Anti-dykddddk
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About This Item
Polecane produkty
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
M1, monoclonal
Postać
buffered aqueous solution
oczyszczone przez
using Protein A
reaktywność gatunkowa
all
stężenie
2-5 mg/mL
metody
western blot: 10 μg/mL
sekwencja immunogenna
DYKDDDDK
Warunki transportu
dry ice
temp. przechowywania
−20°C
Powiązane kategorie
Opis ogólny
The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.
Method of purification - Protein A
Method of purification - Protein A
Immunogen
FLAG; peptide sequence DYKDDDDK
Zastosowanie
Monoclonal ANTI-FLAG® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Działania biochem./fizjol.
Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
Postać fizyczna
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
Uwaga dotycząca przygotowania
Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.
Informacje prawne
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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