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CS1040

Sigma-Aldrich

SIRT1 Assay Kit

sufficient for 100 assays

Synonim(y):

Test aktywności SIRT1

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1 KIT
3570,00 zł

3570,00 zł

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1 KIT
3570,00 zł

About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

3570,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

zastosowanie

sufficient for 100 assays

Poziom jakości

200

Warunki transportu

dry ice

temp. przechowywania

−20°C

informacje o genach

human ... SIRT1(23411)

Opis ogólny

The SIRT1 Assay Kit offers all the reagents required for the measurement of purified SIRT1 activity and for screening of inhibitors/activators.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.

Zastosowanie

SIRT1 Assay Kit has been used in measuring the Sirt1 deacetylase activity in mesenchymal stem cells (MSCs),[1] human retinal endothelial cells (HRECs)[2] and human neuroblastoma SH-SY5Y cells.[3]

Działania biochem./fizjol.

Sirtuins (Sir2) are an evolutionarily conserved family of NAD+ dependent histone/protein deacetylases that tightly couple the cleavage of NAD+ and the deacetylation of protein substrates. The reaction products are nicotinamide, the deacetylated product, and a novel metabolite, 2′-O-acetyl-ADP-ribose. The proteins within this family are named after the first protein discovered from this family, Sir2 (Silent Information Regulator 2). Besides gene silencing, sirtuin proteins are important in other processes such as cell cycling regulation and fatty acid metabolism. SIRT1 is the human homolog of Sir2 and the one most studied to date. SIRT1 mediates p53 dependent process, transcription regulation, muscle differentiation, adipogenesis, and protection from axonal degeneration. SIRT1 also participates in early embryogenesis, neurogenesis, and cardiogenesis.

Cechy i korzyści

The kit offers all the reagents required for the fast and easy measurement of purified SIRT1 activity and for screening of inhibitors/activators. Moreover, the kit contains an inhibitor (nicotinamide) and an activator (resveratrol) as negative and positive controls, respectively.
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • SIRT1 Substrate (Fluorometric) 100 μL
  • Developer Solution 1.5 mL
  • S8446SIRT1 human, recombinant, expressed in E. coli, N-terminal histidine tagged, ≥90% (SDS-PAGE), buffered aqueous glycerol solution 150 μgKarta charakterystyki
  • NAD+ Solution 1 mL

Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

188.6 °F

Temperatura zapłonu (°C)

87 °C

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000261884
0000261884
0000175075
0000175075
0000095999

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SRT1720 SRT1720, CAS 925434-55-5, is a cell-permeable inhibitor of the mitochondrial SIRT3. Inhibition is AceCS2-competitive (Ki = 0.56 µM; Km = 2.44 µM), but NAD+-uncompetitive (Ki = 0.34 µM; Km = 280 µM).

Sigma-Aldrich

567860

SRT1720

Sirtuin 1 human recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

Sigma-Aldrich

SRP0115

Sirtuin 1 human

Anti-SIRT1 antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

S5447

Anti-SIRT1 antibody produced in rabbit

Fenofibrate suppresses cellular metabolic memory of high glucose in diabetic retinopathy via a sirtuin 1-dependent signalling pathway
Zhao S, et al.
Molecular Medicine Reports, 12(4), 6112-6118 (2015)
Nicotinamide phosphoribosyltransferase (Nampt) affects the lineage fate determination of mesenchymal stem cells: a possible cause for reduced osteogenesis and increased adipogenesis in older individuals
Li Y, et al.
Journal of Bone and Mineral Research, 26(11), 2656-2664 (2011)
Jun Zhang et al.
Biochimica et biophysica acta, 1861(7), 1715-1728 (2017-03-17)
Myocardial fibrosis contributes to cardiac dysfunction. Astaxanthin (AST), a member of the carotenoid family, is a well-known antioxidant, but its effect on and underlying mechanisms in myocardial fibrosis are poorly understood. In vivo, myocardial fibrosis and cardiac dysfunction were induced
Nicotinamide phosphoribosyltransferase is essential for IL-1a-mediated dedifferentiation of articular chondrocytes via SIRT1 and ERK complex signaling
Hong,E et al.
The Journal of Biological Chemistry (2011)
Kai Le et al.
International immunopharmacology, 75, 105779-105779 (2019-07-31)
Neonatal hypoxic-ischemic brain injury (HIBI) is a knotty disease that lacks appropriate treatment. Inflammation is an important contributor to brain damage, and microglia are responsible for eliciting early and pronounced inflammatory reactions in the immature brain after hypoxic-ischemic (HI) insult.
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Questions

  1. CAN I USE THE SIRT1 ACTIVITY ASSAY KIT CS1040 TO ESTIMATE SIRT1 ENZYMATIC ACTIVITY IN MICE LUNG TISSUE LYSATE?

    1 answer

    1. The kit is intended to measure purified SIRT1 activity and to screen the efficacy of inhibitors and activators. However, it has also been cited to assess SIRT1 enzymatic activity in cell lysates, including mice tissue lysates. The following citation may be of interest for reference:

      Heliyon. 2024 Sep 4;10(17):e37083.

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