A9207

Avidin−Agarose from egg white

aqueous glycerol suspension

• Synonim(y): Avidin agarose
• Numer MDL: MFCD00130572
• Kod UNSPSC: 41106514
• NACRES: NA.56

Właściwości

• pochodzenie biologiczne: egg white
• Poziom jakości: 200
• białko sprzężone: agarose conjugate
• Formularz: aqueous glycerol suspension
• Matryca: cross-linked 6% beaded agarose
• aktywacja macierzy: epichlorohydrin
• MAR: amino
• spacer macierzy: 15 atoms
• temp. przechowywania: −20°C

Opis

Opis ogólny

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Zastosowanie

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.

Avidin-Agarose from egg white has been used:

• in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
• in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
• in avidin bead assay for purification of labelled biotin
• for capture of glycoproteins from murine cornea samples

Działania biochem./fizjol.

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Definicja jednostki

One unit will bind 1.0 μg of d-biotin.

Postać fizyczna

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable