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S1638

Streptavidin−Agarose from Streptomyces avidinii

buffered aqueous suspension

Synonim(y):

streptavidin agarose beads, streptavidin agarose resin

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Do Państwa/SKUDostępnośćCena netto
1 mL
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
874,00 zł
5 mL
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2960,00 zł

Informacje o tej pozycji

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:

874,00 zł


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form

buffered aqueous suspension

Quality Segment

extent of labeling

≥1 mg per mL

technique(s)

affinity chromatography: suitable

matrix

4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

7 atoms

capacity

≥15 μg/mL binding capacity (biotin)

storage temp.

2-8°C

Application

Streptavidin−agarose from Streptomyces avidinii has been used:
  • to pull down biotinylated cell surface proteins during the quantification of plasma membrane transforming growth factor β (TGFβ) receptor II (TβRII) and Tβ
  • RII internalization
  • in biotinylated miRNA pull-down assay; as secondary antibodies in immunoprecipitation

Streptavidin-agarose is used in protein chromatography, affinity chromatography, and recombinant protein expression and analysis. Streptavidin-agarose has been used to study the oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. Streptavidin-agarose has also been used to develop a method for screening triplex DNA binders from natural plant extracts.
Used for the purification of biotin containing proteins or DNA binding proteins

Biochem/physiol Actions

Streptavidin is a homotetrameric protein, isolated from Streptomyces avidinii, which, like avidin, has a high affinity for biotin. Streptavidin is slightly anionic (pI ~ 5-6) and non-glycosylated. These properties contribute to its relatively low non-specific binding compared to egg white avidin. Streptavidin is also more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin-agarose can be used to immobilize or isolate various biotinylated macromolecules and complexes (proteins, antibodies, lectins, nucleic acids, receptors, and ligands). The inherent high-affinity streptavidin-biotin interaction requires harsh conditions to release biotinylated macromolecules. This feature makes streptavidin-agarose useful in a variety of affinity purification applications.

Physical form

Suspension in 0.01 M sodium phosphate, pH 7.2, containing 0.05 M NaCl and 0.02% sodium azide
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Ta pozycja
11093266910S289011089161001
description

buffered aqueous suspension

description

-

description

lyophilized powder, ≥700 DEA units/mg protein

description

solution, pkg of 1 mL (1,000U)

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

form

buffered aqueous suspension

form

solution

form

lyophilized powder

form

solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

matrix spacer

7 atoms

matrix spacer

-

matrix spacer

-

matrix spacer

-

matrix activation

cyanogen bromide

matrix activation

-

matrix activation

-

matrix activation

-


Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Do the streptavidin beads have disulphide bonds between the dimers?

    1 answer
    1. No analysis of the post-conjugation structure of streptavidin has been performed. This product is tested for binding capacity and functionality. The product S4762 is used in the production of the S1638.

      See the link below for additional information on S4762, streptavidin, including structure details and references:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/896/s4762pis.pdf

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