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D8906

Sigma-Aldrich

Dextran sulfate sodium salt from Leuconostoc spp.

for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

Synonim(y):

6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Manninotriose

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5 G
396,00 zł
10 G
681,00 zł
50 G
2540,00 zł
100 G
4540,00 zł
500 G
14 060,00 zł

396,00 zł


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5 G
396,00 zł
10 G
681,00 zł
50 G
2540,00 zł
100 G
4540,00 zł
500 G
14 060,00 zł

About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352201
NACRES:
NA.31

396,00 zł


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Poproś o zamówienie zbiorcze

pochodzenie biologiczne

synthetic

Poziom jakości

klasa czystości

for molecular biology

Formularz

powder

masa cząsteczkowa

average Mw >500,000 (dextran starting material)

zawiera

0.5-2% phosphate buffer

skład

Sulfur Content, 16.0-19.0%

metody

FISH: suitable
FTIR: suitable

kolor

off-white

przydatny zakres pH

6.0-8 (10 g/L)

rozpuszczalność

H2O: 100 mg/mL

przydatność

suitable for marker for electrophoresis
suitable for nucleic acid hybridization

Zastosowanie

genomic analysis
sample preparation

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Opis ogólny

Dextran sulfate sodium (DSS) is a synthetic sulfated derivative of dextran, a complex polysaccharide composed of glucose molecules. DSS is characterized as a biocompatible, highly branched polysaccharide with 1–6 and 1–4 glycosidic linkages, featuring approximately 2.3 sulfate groups per glucosyl unit. Dextran sulfate is a sulfated polysaccharide with a variable molecular weight ranging from 5 to 1,400 kDa. It finds extensive use in molecular biology and biochemical research, with a particular emphasis on gastrointestinal investigations.DSS is widely recognized for its ability to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD). It is widely recognized for its capacity to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD) by creating models of acute, chronic, and relapsing IBD, by adjusting its concentration and administration frequency. Furthermore, Dextran sulfate is applied in various molecular biology techniques, such as precipitating lipoproteins, accelerating DNA probe hybridization, and facilitating ribosomal isolations. Notably, DSS serves as a valuable cross-linker for chitosan, allowing for the creation of microparticles and nanoparticles used in controlled drug delivery systems. Beyond these applications, DSS plays a central role in diverse research areas, particularly immunology and virology, where it actively modulates immune responses, enhances humoral immunity, influences B-lymphocyte activation, and affects thymocyte reactivity to lectins.

Zastosowanie

Dextran sulfate has been used

  • as a molecular weight marker to assess the purity and homogeneity of the purified polysaccharides by gel electrophoresis[1]
  • as a component of the hybridization buffer in fluorescence in situ hybridization (FISH) protocol for DNA detection and analysis[2][3]
Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Działania biochem./fizjol.

Dextran sulfate, being a sulfated polysaccharide, consistently triggers experimental acute and chronic colitis. It is believed to initiate mucosal injury and inflammation, primarily through a direct toxic impact on epithelial cells. This initial effect activates macrophages and T lymphocytes, ultimately leading to cytokine-mediated cytotoxicity.

Cechy i korzyści

  • Suitable for Nucleic acid hybridization & Molecular Biology
  • Dextran Sulfate with an average molecular weight of >500,000
  • Sulfur Content of 16.0-19.0 %
  • Versatile and adaptable for wide variety of laboratory and research applications

Przydatność

Tested for suitability in nucleic acid hybridizations

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Produkty

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Dostępne procedury, odczynniki i sprzęt do fluorescencyjnej hybrydyzacji in situ (FISH).

Protokoły

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

Siarczany dekstranu jako sole sodowe są rozpuszczalne w wodzie i stabilne, zawierają około 17% siarki do różnych zastosowań.

Powiązane treści

Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, featuring a linear backbone of α-linked d-glucopyranosyl repeating units.

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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