Toluidine Blue is used in various staining procedures, and preparation may vary depending on the specific procedure. For staining frozen sections, aqueous solutions with concentrations ranging from 0.3% to 1% can be used. Additionally, a 0.5% dye in 20% alcohol is suitable for frozen sections. Other solutions may require a specific pH or special preparation.
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Kluczowe dokumenty
89640
Toluidine Blue
8.74% (ZN (THEORY)), for microscopy (Hist., Vit.)
Synonim(y):
Tolonium chloride; Basic Blue 17
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About This Item
Wzór empiryczny (zapis Hilla):
C15H16ClN3S · 0.5ZnCl2
Numer CAS:
Masa cząsteczkowa:
373.97
Numer w indeksie barw:
52040
Beilstein:
4068052
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
NACRES:
MA.02
303,00 zł
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Poproś o zamówienie zbiorcze
Polecane produkty
klasa czystości
for microscopy (Hist., Vit.)
Poziom jakości
Próba
8.74% (ZN (THEORY))
Formularz
powder or crystals
metody
microbe id | staining: suitable
kolor
green to very dark green
rozpuszczalność
methanol: 0.01 g/10 mL, blue to very deep blue
εmax
≥800 at 621-636 nm in water
Zastosowanie
diagnostic assay manufacturing
hematology
histology
temp. przechowywania
room temp
ciąg SMILES
[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
InChI
1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
Klucz InChI
DAQXTSXITPUGRG-UHFFFAOYSA-L
Opis ogólny
Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.
Zastosowanie
Toluidine Blue is suitable for use in the following applications:
- to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
- in metachromatic staining of sulfated mucins and related materials
- as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
- staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)
Działania biochem./fizjol.
Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Are there any instructions on how to mix Toluidine Blue powder (89640) into a staining solution?
1 answer-
Helpful?
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What does C.l. 52040 mean?
1 answer-
The C.I. number is the Colour Index number. This number is globally recognized and is assigned to Dyes and Pigments by the Americal Association of Textile Chemists and Colourists (AATCC) established in 1924. This compound is assigned a C.I. number of 52040. Please see the link below to learn more about the Colour Index program:
https://colour-index.com/Helpful?
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