This product is released based on the testing performed by the Biological Stain Commission. There is no specific solubility specification for this product. At the BSC, the product is dissolved at 50 mg in 250 ml of water for one test, and 100 mg of the dye is dissolved in 150 ml of water for the second test, followed by the addition of additional alcohol. When used to stain frozen sections, a 0.5% solution is typically prepared in 20% alcohol. Some procedures call for dissolving at 10 mg/ml, but this amount of dye cannot always be dissolved. Once dissolved, the solution should be relatively stable.This product does not appear to be hygroscopic, and its solubility can vary somewhat from lot to lot.
Przejdź do
198161
Toluidine Blue O
certified by the BSC
Synonim(y):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
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| Rozmiar/SKU | Dostępność | Cena netto |
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Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C15H16ClN3S
Numer CAS:
Masa cząsteczkowa:
305.83
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
202-146-2
Colour Index Number:
52040
MDL number:
Informacje o cenach i dostępności nie są obecnie dostępne.
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certified by the BSC
Quality Segment
form
powder
composition
Dye content, 80%
color
dark green
λmax
626 nm
ε (extinction coefficient)
≥13000 at 241-247 nm in H2O, ≥28000 at 287-293 nm in H2O, ≥40000 at 625-635 nm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
InChI
1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
InChI key
GEDVVYWLPUPJJZ-UHFFFAOYSA-N
General description
A metachromatic dye suitable for a wide variety of histological staining procedures.
Application
Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.
Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.
Biochem/physiol Actions
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.
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Ta pozycja | |||
|---|---|---|---|
| description certified by the BSC | description certified by the BSC | description powder or crystals, certified by the BSC | description Dye content 90 % |
| color dark green | color dark green | color dark blue | color - |
| form powder | form powder | form powder or crystals | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Dye content, 80% | composition Dye content, ≥82% | composition Dye content, ≥50% | composition Dye content, 90% |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is there a recommendation for dissolving Toluidine Blue (198161-5g lot MKCK0257) at 1 mg/ml in water?
1 answer-
Helpful?
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Could you provide the protocol for using Toluidine Blue for MMA sections?
1 answer-
There are no protocols for using Toluidine Blue O to stain MMA sections.
Helpful?
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