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Merck

198161

Sigma-Aldrich

Toluidine Blue O

certified by the Biological Stain Commission

Synonim(y):

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Wzór empiryczny (zapis Hilla):
C15H16ClN3S
Numer CAS:
Masa cząsteczkowa:
305.83
Numer w indeksie barw:
52040
Numer WE:
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
NACRES:
NA.47

klasa czystości

certified by the Biological Stain Commission

Poziom jakości

Postać

powder

skład

Dye content, 80%

kolor

dark green

λmaks.

626 nm

ε (współczynnik ekstynkcji)

≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O

Zastosowanie

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

ciąg SMILES

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

Klucz InChI

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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Opis ogólny

A metachromatic dye suitable for a wide variety of histological staining procedures.

Zastosowanie

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Działania biochem./fizjol.

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Przydatność

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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