10602400001
Roche
Thrombin
from human plasma
Synonim(y):
coagulation factor Iia, thrombin
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About This Item
Polecane produkty
pochodzenie biologiczne
human plasma
Poziom jakości
Postać
lyophilized
aktywność właściwa
~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)
masa cząsteczkowa
Mr ~33.6 kDa
opakowanie
pkg of 20 U
producent / nazwa handlowa
Roche
optymalne pH
8.2-9.0
Warunki transportu
wet ice
Opis ogólny
Thrombin is a Na+ activated allosteric serine protease. It belongs to chymotrypsin family. Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.
Thrombin is a coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg. The enzyme converts fibrinogen to fibrin. The product contains EDTA and additional stabilizing agents.
Zastosowanie
Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members. It has also been used in platelet spreading on fibrinogen and immunostaining.
Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.
Działania biochem./fizjol.
Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.
Postać fizyczna
Lyophilizate
Uwaga dotycząca przygotowania
Storage conditions (working solution): -15 to -25°C
Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Rekonstytucja
The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Przechowywanie i stabilność
Store at 2 to 8 °C. (Store dry!)
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Oświadczenie o zrzeczeniu się odpowiedzialności
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Inhalation - STOT RE 2
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
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