Przejdź do zawartości
Merck

F3879

Sigma-Aldrich

Fibrinogen from human plasma

50-70% protein (≥80% of protein is clottable)

Synonim(y):

Factor I

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.61

pochodzenie biologiczne

human plasma

Poziom jakości

Postać

powder

jakość

50-70% protein (≥80% of protein is clottable)

masa cząsteczkowa

α-chain 63.5 kDa
β-chain 56 kDa
γ chain 47 kDa (about 4% carbohydrate content)
soluble dimer 340 kDa

stężenie

50-70% protein (biuret)

metody

ELISA: suitable

rozpuszczalność

0.9% NaCl: soluble 10 mg/mL

numer dostępu UniProt

temp. przechowywania

−20°C

informacje o genach

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Opis ogólny

Fibrinogen, or Factor I, is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa

Zastosowanie

Fibrinogen was used in the induction of release of TNF-like cytotoxic factor from murine macrophages. It was used for fabrication of fibrin scaffolds with controlled microscale architecture by a two-photon polymerization-micromolding technique.
Fibrinogen was also used in the development of a fibrinogen-specific sandwich enzyme-linked immunosorbent assay microarray assay for distinguishing between blood plasma and serum samples.
Fibrinogen from human plasma has been used-
  • for the production of fibrin hydrogels
  • for the preparation of fibrin-MSCs (mesenchymal stem cells)-cartilage constructs
  • for analyzing the protein repellent properties of PFDA-co-DEGDME (diethyleneglycol dimethyl ether) surface using Quartz crystal microbalance (QCM)

Działania biochem./fizjol.

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Charakterystyka techniczna

Source material has tested negative for HIV and HBsAg.

Postać fizyczna

Lyophilized powder containing ~15% sodium citrate and ~25% sodium chloride.

Rekonstytucja

The optimal way to solubilize fibrinogen is to layer it on top of warm (37 ºC) saline, as fibrinogen will not dissolve in water. The saline concentration can be in the range of 0.85-0.9%. The fibrinogen-saline solution can be gently agitated, but it must not be vortexed. The fibrinogen will slowly dissolve to give a hazy solution. Fibrinogen may be sterile-filtered, but may not go through a 0.1 μ filter. A 0.2 μ filter is suggested, with positive pressure using a syringe and "button" filter. Vacuum filtration should not be used, since this will lead to breakdown of the molecule during the filtration.

Komentarz do analizy

Protein determined by biuret

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Amphiphilic Copolymer Coatings via Plasma Polymerisation Process: Switching and Anti-Biofouling Characteristics.
Kumar V, et al.
Plasma Processes and Polymers, 8, 373-385 (2011)
Anastasia Koroleva et al.
Biofabrication, 4(1), 015001-015001 (2012-01-20)
Fabrication of three-dimensional (3D) fibrin scaffolds with tightly controllable pore sizes and interconnections has been investigated. The scaffolds were produced using a combination of two-photon polymerization (2PP) and micromolding techniques. Master structures were fabricated by 2PP and regenerated in fibrin
T Kajikawa et al.
Journal of biological response modifiers, 6(1), 88-95 (1987-02-01)
The triggering activities of heterologous fibrinogen and fibrin on endogenous production of tumor necrosis factor (TNF)-like cytotoxic factor in vivo were examined. The triggering activities of fibrinogen or fibrin from four species injected into the peritoneal cavity of C3H/He mice
Chia-Chun Chen et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 30(3), 393-400 (2012-01-24)
Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were
L J Walsh et al.
Journal of dental research, 65(12), 1424-1426 (1986-12-01)
T6 is an antigen which is a highly specific marker for Langerhans cells. Previous studies have demonstrated that Interleukin-1 (IL-1) and an IL-1 inhibitor (ILS) modulate T6 expression (T6E) in explant culture. The present study examined the effects of IL-1

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej