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10476498001
Roche
Luciferase
from Photobacterium fischeri
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About This Item
Polecane produkty
pochodzenie biologiczne
bacterial (Photobacterium fischeri)
opakowanie
pkg of 2 mg
producent / nazwa handlowa
Roche
optymalne pH
6.8
Warunki transportu
wet ice
Opis ogólny
Contents: Lyophilizate
Specific activity: Approximately 15 mU/mg protein at +25°C with FMN and myristine aldehyde as the substrates, and 50 mU NAD(P)H:FMN oxidoreductase per ml assay solution (based on the relative light emission in the ATP system under the formation of pyrophosphate with 1 mU luciferase from Photinus pyralis).
Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)
Luciferase from bacteria exists as a heterodimer with α and β chains.
Zastosowanie
Luciferase may be used:
- to treat mammary tumor cryosections for bioluminescence imaging studies
- as a component of luciferase reaction mixture
- in bioluminescence assay membrane preparations of Zymomonas mobilis
Działania biochem./fizjol.
Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.
Jakość
Contaminants: <10 mU NADH:FMN oxidoreductase/mg protein, <10 mU myokinase/mg protein, and <1 mU NADH oxidase/mg protein
Przechowywanie i stabilność
Store at 2 to 8 °C. (A decrease in activity of approximately 20% may occur within 12 months.)
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
No data available
Temperatura zapłonu (°C)
No data available
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Produkty
Lucyferaza Firefly jest czułym reporterem do badań genów ze względu na jej brak w komórkach lub tkankach ssaków.
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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