MABE647
Anti-acetyl-Histone H3 (Lys27) Antibody, clone RM172
clone RM172, from rabbit
Synonim(y):
Histone H3.3, Histone H3
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Wybierz wielkość
100 μG
2720,00 zł
Wybierz wielkość
Zmień widok
100 μG
2720,00 zł
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
RM172, monoclonal
reaktywność gatunkowa
human
metody
multiplexing: suitable
western blot: suitable
izotyp
IgG
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
acetylation (Lys27)
informacje o genach
human ... H3F3B(3021)
Opis ogólny
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
Specyficzność
This antibody reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in Histone H3.
Immunogen
A linear peptide corresponding to human Histone H3 acetylated at Lys27.
Zastosowanie
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This Anti-acetyl-Histone H3 (Lys27) Antibody, clone RM172 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.
Jakość
Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 1 μg/mL of this antibody detected acetyl-Histone H3 (Lys27) in sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 1 μg/mL of this antibody detected acetyl-Histone H3 (Lys27) in sodium butyrate treated HeLa acid extract.
Opis wartości docelowych
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Postać fizyczna
Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Michael S Hoetker et al.
Nature cell biology, 25(8), 1121-1134 (2023-07-18)
The epigenetic mechanisms that maintain differentiated cell states remain incompletely understood. Here we employed histone mutants to uncover a crucial role for H3K36 methylation in the maintenance of cell identities across diverse developmental contexts. Focusing on the experimental induction of
Steven J Wu et al.
Nature biotechnology, 39(7), 819-824 (2021-04-14)
Methods for quantifying gene expression1 and chromatin accessibility2 in single cells are well established, but single-cell analysis of chromatin regions with specific histone modifications has been technically challenging. In this study, we adapted the CUT&Tag method3 to scalable nanowell and
Chi Sun et al.
Scientific reports, 13(1), 12899-12899 (2023-08-10)
Enhancers function with a basal promoter to control the transcription of target genes. Enhancer regulatory activity is often studied using reporter-based transgene assays. However, unmatched results have been reported when selected enhancers are silenced in situ. In this study, using
Marit W Vermunt et al.
Molecular cell, 83(5), 715-730 (2023-03-04)
Transcriptional enhancers have been extensively characterized, but cis-regulatory elements involved in acute gene repression have received less attention. Transcription factor GATA1 promotes erythroid differentiation by activating and repressing distinct gene sets. Here, we study the mechanism by which GATA1 silences
Silvia Pomella et al.
Nature communications, 14(1), 8373-8373 (2023-12-16)
Rhabdomyosarcomas (RMS) are pediatric mesenchymal-derived malignancies encompassing PAX3/7-FOXO1 Fusion Positive (FP)-RMS, and Fusion Negative (FN)-RMS with frequent RAS pathway mutations. RMS express the master myogenic transcription factor MYOD that, whilst essential for survival, cannot support differentiation. Here we discover SKP2
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