AB5539

Anti-Neurofilament H (200 kDa) Antibody

CHEMICON^®, chicken polyclonal

• Synonim(y): NF-H
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat, pig, bovine, feline, human, mouse
• Application: ICC, IHC (p), WB
• Citations: 192

Właściwości

• Nazwa produktu: Anti-Neurofilament H Antibody, Chemicon^®, from chicken
• biological source: chicken
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, pig, bovine, feline, human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• NCBI accession no.: NM_021076.2
• UniProt accession no.: P12036
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Nefh(528842) ; cat ... Nefh(101087272) ; human ... NEFH(4744) ; mouse ... Nefh(380684) ; pig ... Nefh(100156492) ; rat ... Nefh(24587)

Opis

General description

200 kDa

Immunogen

Purified bovine NF-H.

Application

Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.

Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.

Physical form

Format: Purified

Purified chicken IgY in PBS containing 5mM sodium azide.

Analysis Note

Control
Positive control: brain.

Negative control: kidney, liver.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable