MAB5448
Anti-Neurofilament NF-H Antibody, clone TA51
culture supernatant, clone TA51, Chemicon®
Synonim(y):
NF-H
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
TA51, monoclonal
reaktywność gatunkowa
human, rat, hamster, monkey
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NEFH(4744)
rat ... Nefh(24587)
rhesus monkey ... Nefh(717705)
Specyficzność
Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}.
Immunogen
Epitope: carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH
Zastosowanie
Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g
for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}
Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.
Optimal working dilutions must be determined by end user.
for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}
Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.
Optimal working dilutions must be determined by end user.
Postać fizyczna
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Przechowywanie i stabilność
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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