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268298

DAPI, Dihydrochloride

Name: DAPI, Dihydrochloride
Brand: MM
Price: 989 PLN

Cell-permeable DNA-binding dye.

Synonim(y):

DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl

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Gabaryty przesyłki	SKU	Dostępność	Cena netto
10 mg		Sprawdź dostępność w koszyku	989,00 zł

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):

C₁₆H₁₅N₅ · 2HCl

Numer CAS:

28718-90-3

Masa cząsteczkowa:

350.25

UNSPSC Code:

12352200

NACRES:

NA.54

MDL number:

MFCD00012681

989,00 zł

Sprawdź dostępność w koszyku

Pomoc techniczna

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Właściwości

Quality Segment

100

description

RTECS - NL5995050

assay

≥90% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

yellow

solubility

water: 5 mg/mL, ethanol: soluble, methanol: soluble

fluorescence

λ_ex ~359 nm, λ_em ~461 nm

shipped in

ambient

storage temp.

−20°C

SMILES string

[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Opis

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Binds preferentially to DNA rich in adenine and thymine

Product does not compete with ATP.

Reversible: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Ta pozycja	D9542	D8417	MBD0015
Sigma-Aldrich 268298 DAPI, Dihydrochloride	Sigma-Aldrich D9542 DAPI	Sigma-Aldrich D8417 4′,6-Diamidino-2-phenylindole dihydrochloride	Sigma-Aldrich MBD0015 DAPI ready made solution
color yellow	color -	color -	color -
fluorescence λ_ex ~359 nm, λ_em ~461 nm	fluorescence λ_ex 340 nm; λ_em 488 nm (nur DAPI), λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)	fluorescence λ_ex 340 nm; λ_em 488 nm (nur DAPI), λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)	fluorescence λ_ex 340 nm; λ_em 488 nm (nur DAPI), λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
form solid	form powder	form powder	form liquid
solubility water: 5 mg/mL, methanol: soluble	solubility H₂O: 20 mg/mL, PBS: insoluble	solubility H₂O: 20 mg/mL, PBS: insoluble	solubility water: soluble
Quality Level 100	Quality Level 300	Quality Level 200	Quality Level 200
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

pictograms

GHS07

signalword

Warning

hcodes

H315,H317,H335

pcodes

P261 - P264 - P271 - P272 - P280 - P302 + P352

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certyfikaty analizy (CoA)

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Numer pozycji handlu globalnego

SKU	NUMER GTIN
268298-10MG	04055977197785

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