06-600

Anti-NR2B Antibody

Upstate^®, from rabbit

• Synonim(y): N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 2B, N-methyl-D-aspartate receptor subunit 3, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: mouse
• reaktywność gatunkowa (przewidywana na podstawie homologii): monkey (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), human (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• producent / nazwa handlowa: Upstate^®
• metody: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NM_008171
• numer dostępu UniProt: Q13224
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: bovine ... Grin2B(537804) ; chimpanzee ... Grin2B(473373) ; dog ... Grin2B(494009) ; human ... GRIN2B(2904) ; mouse ... Grin2B(14812) ; rat ... Grin2B(24410) ; rhesus monkey ... Grin2B(698571)

Opis

Opis ogólny

NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer′s disease, epilepsy and ischemic neuronal cell death.

Specyficzność

NR2B; the immungen sequence is identical in mouse, rat, human, bovine, dog and chimp NR2B. Human NR2B is sometimes referred to as hNR3 in the literature.

Immunogen

a.a. 1437-1456 of mature mouse NR2B or 1463-1482 of NR2B precursor; signal is a.a. residues 1-26 [KFNGSSNG HVYEKLSSIESDV]. This sequence is identical to a.a. 1437-1456 of mature rat NR2B and a.a. 1465-1484 of human NR3, containing a N-terminal lysine.

Zastosowanie

Detect NR2B using this Anti-NR2B Antibody validated for use in IC, IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases

Jakość

Routinely evaluated by Western Blot on rat brain microsomal preparation (Catalog # 12-144).

Western Blot Analysis:
0.5-2 μg/mL of this lot of antibody detected NR2B (~180 kDa) in rat brain microsomal preparation (Catalog # 12-144).

Opis wartości docelowych

180 kDa

Postać fizyczna

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, and 0.05% sodium azide. Frozen solution.

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Komentarz do analizy

Control
Mouse brain tissue

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1