05-432
Anti-NR1 Antibody, CT
Upstate®, from mouse
Synonim(y):
Grin1, NMDA R1 receptor C1 cassette, glutamate receptor, ionotropic, N-methyl D-aspartate 1
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Wybierz wielkość
200 μG
2610,00 zł
Wybierz wielkość
Zmień widok
200 μG
2610,00 zł
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
monoclonal
application:
ICC
IP
WB
IP
WB
reaktywność gatunkowa:
rat
metody:
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
61
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
monoclonal
reaktywność gatunkowa
rat
reaktywność gatunkowa (przewidywana na podstawie homologii)
human, mouse
producent / nazwa handlowa
Upstate®
metody
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... GRIN1(2902)
rat ... Grin1(24408)
Opis ogólny
NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
Specyficzność
This antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.
Immunogen
Epitope: The epitope maps to a region (a.a. 834-864) common to all NR1 isoforms
His-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.
Zastosowanie
Anti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.
Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.
Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.
Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.
Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.
Jakość
Routinely evaluated by Western Blot on rat brain microsomal protein preparation.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).
Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).
Opis wartości docelowych
130 kDa observed
Postać fizyczna
Format: Purified
Purified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2° to 8ºC from date of receipt.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.
She, K; Ferreira, JS; Carvalho, AL; Craig, AM
The Journal of Biological Chemistry null
NMDA receptors mediate synaptic competition in culture.
She, K; Craig, AM
Testing null
Natural reward experience alters AMPA and NMDA receptor distribution and function in the nucleus accumbens.
Pitchers, KK; Schmid, S; Di Sebastiano, AR; Wang, X; Laviolette, SR; Lehman, MN; Coolen, LM
Testing null
Jia-Hua Hu et al.
Nature neuroscience, 15(6), 836-844 (2012-05-09)
Group I metabotropic glutamate receptors (mGluRs), including mGluR1 and mGluR5, are G protein–coupled receptors (GPCRs) that are expressed at excitatory synapses in brain and spinal cord. GPCRs are often negatively regulated by specific G protein–coupled receptor kinases and subsequent binding
Kourtney Graham et al.
NPJ schizophrenia, 5(1), 1-1 (2019-01-16)
The 14-3-3 family of proteins is genetically linked to several psychiatric disorders, including schizophrenia. Our 14-3-3 functional knockout (FKO) mice, as well as other 14-3-3 knockout models, have been shown to exhibit behavioral endophenotypes related to schizophrenia. While specific forebrain
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