The contents of the Tris base are added to the phenol:chloroform:isoamyl alcohol mixture, and the contents are well mixed. The amount added is 6.5 mL equilibration buffer per 100 mL phenol, designed so that only one treatment is necessary to raise the pH. Allow the mixture to separate, which may take 2-4 hours. The upper aqueous phase is removed and discarded. The remaining lower organic phase is divided into aliquots and stored at -20 °C. The solution should be stable for up to 12 months. The pH of the phenol should be raised from 6.7 to about 7.9-8. Only one treatment is needed.
价格与库存信息目前不能提供
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等级
for molecular biology
方案
≥99.0%
表单
liquid
pH值（酸碱度）
6.5-6.9(pH of phenolic phase)
7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)
应用
agriculture
储存温度
2-8°C
应用
对于需要更高 pH 值的应用，例如大的完整基因组 DNA的分离，建议添加平衡缓冲液。
包装
随配 P3803 和一瓶单独的平衡缓冲液，用于将苯酚相调节至 pH 8.0 ± 0.2。
适用性
这批苯酚：氯仿：异戊醇 25:24:1 采用从大肠杆菌中提取 的pBR322 DNA 的粗制品进行了测试。 根据琼脂糖凝胶电泳评估，提取的 DNA 没有降解或浓度降低。 采用相同的操作程序处理纯化的 pBR322 DNA 和 λ Hind III 消化 DNA。 根据琼脂糖凝胶电泳评估，纯化的制剂没有检测到降解或浓度降低。
制备说明
将小瓶平衡缓冲液的全部内容物添加到大瓶苯酚：氯仿：异戊醇中。 在使用前轻柔地混合并使相实现分离，大约 2-4 小时。 这会将苯酚溶液的 pH 值从 6.7± 0.2 调整到 8.0± 0.2。
其他说明
添加平衡缓冲液后，产品可稳定 6 个月，而不添加缓冲液可稳定 2 年。 在添加平衡缓冲液后，在 -20 °C 下避光储存可将保质期延长至 1 年以上。
相关产品
产品编号
说明
价格
警示用语：
Danger
危险分类
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
靶器官
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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实验方案
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
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Hello, I used P2069 for DNA extraction from mouse tails. After adding the equilibration buffer, I wait for 2~4hrs until phase seperation. At this point, I have a question. I heard that lower phase is the phase I utilize, so Can I aliquot the lower phase?
1 answer-
Helpful?
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Hello, I used this material for DNA extraction from mouse tail. After mixing the equilibration buffer, there was two phase. Which phase should I use? Also, Can I aliquot the liquid?
1 answer-
The lower phase should be used for DNA extraction. It is possible to aliquot the liquid immediately after mixing, before the phases separate.
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After equilibrating with the supplied Tris buffer, can I aliquot this for use, if so, should I just top each aliquot with additional Tris pH 8?
1 answer-
Please see the link below to review the product datasheet. Note that the prepration instructions require the addition of the entire vial (6.5 mL) of equilabration buffer to the contents of the product. Aliquot immediately after the gentle mixing step. During the 2 - 4 hour incubation period the mixure will phase separate. At this point the aliquots may be stored for long term use according to the instructions.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/144/892/p2069dat.pdfHelpful?
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There is an Equilibration Buffer (Product B5658) that is included with Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1. Is B5658 also available separately?
1 answer-
No, it is only available as part of P2069.
Helpful?
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I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?
1 answer-
In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.
Helpful?
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I ordered Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; I got one bottle of the Phenol:Chloroform:Isoamyl Alcohol and one bottle of B5658, Equilibration buffer. When and how am I supposed to use the B5658?
1 answer-
B5658 is a Tris buffer that is used to raise the pH of the phenol:chloroform:isoamyl alcohol mixture, to make it suitable for isolation of genomic DNA. The contents of the two bottles need to be well mixed. Then allow the mixture to separate; this may take 2-4 hours. This procedure should raise the pH of the phenol phase from 6.7 to about 8.0. Only one "treatment" is needed. This solution should be stable for up to 12 months.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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There are two layers in the bottle of Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; which layer should I use?
1 answer-
The lower phase is the phase that is to be used.
Helpful?
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