Product P1944 - Phenol:Chloroform: Iso-amyl alcohol (125:24:1) is acidic. As such, the RNA isolates in an acidic pH and will partition the genomic DNA into the organic phase leaving only the RNA in the top aqueous phase.
价格与库存信息目前不能提供
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等级
Molecular Biology
for molecular biology
方案
≥99.0%
表单
liquid
pH值（酸碱度）
4.3-4.7
应用
life science and biopharma
储存温度
2-8°C
一般描述
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.[1]
应用
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
靶器官
Liver,Kidney, Nervous system,Kidney,Liver,Skin
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
175.0 °F - closed cup
闪点(°C)
79.44 °C - closed cup
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Hello, Your product is fractionated into two phases. Should I use lower phase for RNA extraction? Thank you
1 answer-
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