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Merck
  • A starch-binding domain identified in α-amylase (AmyP) represents a new family of carbohydrate-binding modules that contribute to enzymatic hydrolysis of soluble starch.

A starch-binding domain identified in α-amylase (AmyP) represents a new family of carbohydrate-binding modules that contribute to enzymatic hydrolysis of soluble starch.

FEBS letters (2014-03-13)
Hui Peng, Yunyun Zheng, Maojiao Chen, Ying Wang, Yazhong Xiao, Yi Gao
要旨

A novel starch-binding domain (SBD) that represents a new carbohydrate-binding module family (CBM69) was identified in the α-amylase (AmyP) of the recently established alpha-amylase subfamily GH13_37. The SBD and its homologues come mostly from marine bacteria, and phylogenetic analysis indicates that they are closely related to the CBM20 and CBM48 families. The SBD exhibited a binding preference toward raw rice starch, but the truncated mutant (AmyPΔSBD) still retained similar substrate preference. Kinetic analyses revealed that the SBD plays an important role in soluble starch hydrolysis because different catalytic efficiencies have been observed in AmyP and the AmyPΔSBD.

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製品内容

Sigma-Aldrich
α-アミラーゼ, 熱安定型, solution, for use in Total Dietary Fiber Assay, TDF-100A
Sigma-Aldrich
α-アミラーゼ from human saliva, Type XIII-A, lyophilized powder, 300-1,500 units/mg protein
Sigma-Aldrich
α-アミラーゼ ブタ膵臓由来, Type VI-B, ≥5 units/mg solid
Sigma-Aldrich
α-アミラーゼ from Aspergillus oryzae, powder, ~30 U/mg
Sigma-Aldrich
タカジアスターゼ from Aspergillus oryzae, powder, slightly beige, ~100 U/mg
Sigma-Aldrich
α-アミラーゼ from Aspergillus oryzae, ≥150 units/mg protein (biuret)
Sigma-Aldrich
α-アミラーゼ Bacillus licheniformis由来, Type XII-A, saline solution, ≥500 units/mg protein (biuret)
Sigma-Aldrich
α-アミラーゼ Bacillus sp.(バシラス属)由来, Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)
Sigma-Aldrich
α-アミラーゼ from human saliva, Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein
Sigma-Aldrich
α-アミラーゼ Bacillus subtilis(枯草菌)由来, powder, yellow-brown, ~50 U/mg
Sigma-Aldrich
α-アミラーゼ Bacillus licheniformis由来, lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE)
Sigma-Aldrich
α-アミラーゼ from Aspergillus oryzae, powder, ~1.5 U/mg (~0.2 U acc. to Willstätter)
Sigma-Aldrich
α-アミラーゼ Bacillus sp.(バシラス属)由来, liquid
Sigma-Aldrich
α-アミラーゼ ブタ膵臓由来, PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)
Sigma-Aldrich
α-アミラーゼ Bacillus sp.(バシラス属)由来, powder, ≥400 units/mg protein (Lowry)
Sigma-Aldrich
α-アミラーゼ from Aspergillus oryzae, aqueous solution, ≥800 FAU/g
Sigma-Aldrich
α-アミラーゼ Bacillus amyloliquefaciens由来, liquid, ≥250 units/g
Sigma-Aldrich
α-アミラーゼ Bacillus subtilis(枯草菌)由来, powder, yellow-brown, ~380 U/mg
Sigma-Aldrich
α-アミラーゼ ブタ膵臓由来, Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)
Sigma-Aldrich
デンプン, puriss. p.a., from potato, reag. ISO, reag. Ph. Eur., soluble
Sigma-Aldrich
デンプン ジャガイモ由来, Soluble
Sigma-Aldrich
デンプン トウモロコシ由来
Sigma-Aldrich
デンプン ジャガイモ由来, Powder
Sigma-Aldrich
デンプン トウモロコシ由来, practical grade
Sigma-Aldrich
デンプン イネ由来
Sigma-Aldrich
デンプン, from potato, tested according to Ph. Eur.
Sigma-Aldrich
デンプン トウモロコシ由来
Sigma-Aldrich
デンプン from wheat, Unmodified
Sigma-Aldrich
デンプン ジャガイモ由来, suitable for electrophoresis
Supelco
デンプン トウモロコシ由来, analytical standard, analytical standard for Starch Assay Kits SA-20 and STA-20