10069

α-アミラーゼ Bacillus subtilis（枯草菌)由来

powder, yellow-brown, ~380 U/mg

• CAS番号: 9000-90-2
• Enzyme Commission number: 3.2.1.1 (BRENDA, IUBMB)
• EC Number: 232-565-6
• MDL番号: MFCD00081319
• UNSPSCコード: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

特性

• 由来生物: Bacillus sp.
• フォーム: powder
• 比活性: ~380 U/mg
• 環境により配慮した代替製品の特徴: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 色: yellow-brown
• 環境により配慮した代替製品カテゴリ: , Enabling
• 保管温度: 2-8°C

詳細

詳細

α-Amylase (α-1,4-glucan-4-glucanohydrolase) belongs to the glycosyl hydrolase family 13. The two aspartic residues and one glutamic acid residue are the prime catalytic residues of α-amylase.[1] All amylases have three domain regions, namely, domain A with a central (β/α)8 barrel, domain B, and β-structure with a Greek key motif encompassing domain C.[1]

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

アプリケーション

α-Amylase from Bacillus sp. has been used:

• as a dispersal enzyme to test degradation of S. aureus biofilms[2],
• in the enzymatic hydrolysis of tapioca starch[3]
• in the enzymolysis of plant-based native and the amorphous granular starches[4]

Heat stability of bacterial α-amylasescitation; Action pattern on sweet potato starch, amylose and amylopectincitation; Action on native wheat starch.citation

生物化学的／生理学的作用

α-Amylase mediates the hydrolysis of starch, malto-oligosaccharides, and glycogen at the α-D-(1,4)-glucosidic linkages.[1] Bacillus sp. serve as an important cell factory for the heterogeneous production of α-amylase.[5] An extracellular secreted thermostable amylase from the Bacillus subtilis strain has also been reported.[6]

単位の定義

1 unitは、基質として製品番号 85642を使用し、pH 6.9、25°C、1分間で、1 μmolのマルトースを遊離させる酵素量に相当します。

その他情報

細菌由来α-アミラ-ゼの熱安定性[7], サツマイモデンプン, アミロ-ス, アミロペクチンに対する作用パタ-ン[8], 天然型コムギデンプンに対する作用[9]。

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): dust mask type N95 (US), Eyeshields, Faceshields, Gloves