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  • Notch regulation of progenitor cell behavior in quiescent and regenerating auditory epithelium of mature birds.

Notch regulation of progenitor cell behavior in quiescent and regenerating auditory epithelium of mature birds.

Developmental biology (2008-11-18)
Nicolas Daudet, Robin Gibson, Jialin Shang, Amy Bernard, Julian Lewis, Jennifer Stone
要旨

Unlike mammals, birds regenerate auditory hair cells (HCs) after injury. During regeneration, mature non-sensory supporting cells (SCs) leave quiescence and convert into HCs, through non-mitotic or mitotic mechanisms. During embryogenesis, Notch ligands from nascent HCs exert lateral inhibition, restricting HC production. Here, we examined whether Notch signaling (1) is needed in mature birds to maintain the HC/SC pattern in the undamaged auditory epithelium or (2) governs SC behavior once HCs are injured. We show that Notch pathway genes are transcribed in the mature undamaged epithelium, and after HC injury, their transcription is upregulated in the region of highest mitotic activity. In vitro treatment with DAPT, an inhibitor of Notch activity, had no effect on SCs in the undamaged epithelium. Following HC damage, DAPT had no direct effect on SC division. However, after damage, DAPT caused excessive regeneration of HCs at the expense of SCs, through both mitotic and non-mitotic mechanisms. Conversely, overexpression of activated Notch in SCs after damage caused them to maintain their phenotype and inhibited HC regeneration. Therefore, signaling through Notch is not required for SC quiescence in the healthy epithelium or to initiate HC regeneration after damage. Rather, Notch prevents SCs from regenerating excessive HCs after damage.

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Sigma-Aldrich
ジメチルスルホキシド, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
ジメチルスルホキシド, ACS reagent, ≥99.9%
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ジメチルスルホキシド, for molecular biology
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ジメチルスルホキシド, suitable for HPLC, ≥99.7%
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ジメチルスルホキシド, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
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ジメチルスルホキシド, ReagentPlus®, ≥99.5%
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ジメチルスルホキシド, ≥99.5% (GC), suitable for plant cell culture
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ジメチルスルホキシド, puriss. p.a., ACS reagent, ≥99.9% (GC)
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ジメチルスルホキシド, BioUltra, for molecular biology, ≥99.5% (GC)
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ジメチルスルホキシド, PCR Reagent
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ジメチルスルホキシド, puriss. p.a., dried, ≤0.02% water
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ジメチルスルホキシド, anhydrous, ≥99.9%
SAFC
Minimum Essential Medium, with Earle′s Balanced Salts, with 2.0 mM L-glutamine, with non-essential amino acids, without sodium bicarbonate, dry powder, suitable for cell culture
SAFC
Minimum Essential Medium, with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture
SAFC
Minimum Essential Medium, with Earle′s salts, with 2.0 mM L-glutamine, with 20.0 mM HEPES, with non-essential amino acids, liquid, sterile-filtered, suitable for cell culture
SAFC
Minimum Essential Medium, with Earle′s Balanced Salts, with non-essential amino acids, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
ジメチルスルホキシド, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
ジメチルスルホキシド, ≥99.5%
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ジメチルスルホキシド, JIS special grade, ≥99.0%
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Minimum Essential Medium, with Earle′s Balanced Salts, with 25mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Supelco
ジメチルスルホキシド, analytical standard
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ジメチルスルホキシド, suitable for HPLC
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ジメチルスルホキシド, SAJ first grade, ≥99.0%
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ジメチルスルホキシド, ≥99.0%, suitable for absorption spectrum analysis