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出荷可能日2025年4月25日詳細
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製品名
Ascentis® Express C18, 2.7 µm HPLCカラム, 2.7 μm particle size, L × I.D. 10 cm × 3 mm
物質
stainless steel column
品質水準
認証
suitable for USP L1
製品種目
Ascentis®
特徴
endcapped
メーカー／製品名
Ascentis®
包装
1 ea of
パラメーター
60 °C temp. range
600 bar max. pressure (9000 psi)
テクニック
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × 内径
10 cm × 3 mm
表面積
135 m2/g
不純物
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
マトリックス活性基
C18 (octadecyl) phase
粒径
2.7 μm
ポアサイズ
90 Å
使用pH
2-9
アプリケーション
food and beverages
分離法
reversed phase
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関連するカテゴリー
詳細
Ascentis Express HPLC カラムは、Fused-Core® 粒子技術により、低背圧を維持しながら、サブ-2 μm 粒子を高速、且つ高効率で提供することができます。高効率と低背圧の組合せがUPLC® (又は他の超高圧システム) のユーザー、並びに従来型HPLCのユーザーの助けになります。
この新カラム技術についての更なる情報については、Ascentis Expressのホームページにアクセスを。
この新カラム技術についての更なる情報については、Ascentis Expressのホームページにアクセスを。
アプリケーション
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
アプリケーション
製品番号
詳細
価格
ガードカートリッジ
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
53814-U:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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資料
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
Accurate steroid analysis in blood serum using Supel™ Swift HLB DPX Tips; UTAK and NIST standards.
プロトコル
Analysis of banned neonicotinoid insecticides from dandelion blossoms using QuEChERS and LC-MS.
タンポポの花からの禁止されたネオニコチノイド系殺虫剤のQuEChERSおよびLC-Mによる分析。
Chromatograms
application for HPLCapplication for HPLCapplication for HPLCapplication for HPLC表示を増やす-
What is the Department of Transportation shipping information for this product?
1 回答-
役に立ちましたか？
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 回答-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
役に立ちましたか？
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 回答-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
役に立ちましたか？
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Can I use Ascentis Express on any type of HPLC system?
1 回答-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
役に立ちましたか？
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Can I use Ascentis Express on a UHPLC system?
1 回答-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
役に立ちましたか？
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 回答-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
役に立ちましたか？
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 回答-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
役に立ちましたか？
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 回答-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
役に立ちましたか？
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 回答-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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