W3144

Wortmannin

from Penicillium funiculosum, ≥95% (HPLC), solution, PI3-kinase inhibitor

• 別名: 抗生物質SL-2052
• 実験式（ヒル表記法）: C₂₃H₂₄O₈
• CAS番号: 19545-26-7
• 分子量: 428.43
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• product name: ワートマニン、調製済溶液, from Penicillium funiculosum, ≥95% (HPLC)
• 由来生物: Penicillium funiculosum
• 品質水準: 200
• アッセイ: ≥95% (HPLC)
• 保管条件: protect from light
• 濃度: 10 mM in DMSO (0.2 μm filtered)
• 輸送温度: wet ice
• 保管温度: −20°C
• InChI: 1S/C23H24O8/c1-10(24)30-13-7-22(2)12(5-6-14(22)25)16-18(13)23(3)15(9-28-4)31-21(27)11-8-29-20(17(11)23)19(16)26/h8,12-13,15H,5-7,9H2,1-4H3/t12-,13+,15+,22-,23-/m0/s1
• InChI Key: QDLHCMPXEPAAMD-QAIWCSMKSA-N
• 遺伝子情報: human ... PIK3CD(5293) , PIK3CG(5294) , PIK3R1(5295)

詳細

アプリケーション

Wortmannin, Ready Made Solution has been used:

• as a phosphoinositide 3-kinase (PI3K) pathway inhibitor
• as an endoderm-inducing agent in stem cell selection and differentiation
• as a autophagy inhibitor

Wortmannin, a low molecular weight membrane permeable hydrophobic fungal metabolite, is widely used to study and verify the involvement of phosphoinosidite 3-kinase (PI3K)/Akt in cell signaling cascades that regulate key processes such as cell survival and apoptosis. Wortmannin may also be used to block and study the functional roles of polo-like kinases involved in the regulation of mitosis.

生物化学的／生理学的作用

ワートマニンは、Penicilium fumiculosumが産生する分子量の低い疎水性の真菌代謝産物であり、ステロール様構造をもちます。ワートマニンでPI3K/Aktシグナル伝達カスケードを阻害すると、放射線または血清枯渇によるアポトーシス作用が促進され、サイトカインの抗アポトーシス作用が遮断されます。また、PI3Kを阻害するとインスリンレセプターの活性化で誘導される短期の代謝作用の多くが遮断されます。ワートマニンは、重要な制御因子であるポロ様キナーゼ(Plk)ファミリーのPlk1とPlk3のふたつの酵素による有糸分裂を阻害することが研究によって示されています。

Wortmannin is a low-molecular-weight hydrophobic fungal metabolite with a sterol-like structure produced by Penicillium funiculosum. It is a selective inhibitor of phosphoinosidite 3-kinase. It interferes with Akt signal transduction cascade, enhancing the apoptotic effects of radiation or serum withdrawal and blocks the antiapoptotic effect of cytokines. Wortmannin is membrane-permeable, thus facilitating whole-cell studies of G-protein-coupled receptor and receptor tyrosine kinase signalling pathways. PI3K inhibition by wortmannin also blocks many of the short-term metabolic effects induced by insulin receptor activation. Research has demonstrated that wortmannin inhibits two mitotic enzymes, key regulators in the Polo-like kinase (Plk) family, Plk1 and Plk3.

Wortmannin possesses anti-inflammatory properties. This mold metabolite is capable of preventing an intermediate step in transcytosis. In fisher rat thyroid (FRT) cells, wortmannin helps to block bidirectional transcytosis of ricin.

特徴および利点

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物理的形状

ワートマニンの調製済み溶液です。10 mMの0.2μmフィルター滅菌済みDMSO溶液です。

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302 + H332
• 注意書き: P261 - P264 - P270 - P271 - P301 + P312 - P304 + P340 + P312
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): 188.6 °F - closed cup
• 引火点(℃): 87 °C - closed cup