Stock solutions remain stable for a period of up to two weeks when stored at 4°C.
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由来生物
human plasma
品質水準
詳細
Iron-saturated
製品種目
BioReagent
アッセイ
≥97%
フォーム
powder
テクニック
cell culture | mammalian: suitable
不純物
HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods)
endotoxin, tested
溶解性
H2O: 50 mg/mL
UniProtアクセッション番号
輸送温度
ambient
保管温度
2-8°C
遺伝子情報
human ... TF(7018)
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アプリケーション
トランスフェリンは、血清中に存在する主要な鉄結合細胞送達分子です。無血清細胞培養系では、鉄輸送因子が必要です。細胞は、トランスフェリンと結合した鉄を、細胞表面のトランスフェリン受容体を介して、生理学的に適切な方法で処理するため、トランスフェリンは主要な鉄輸送因子とされています。ヒトホロトランスフェリンは、広範な細胞型に使用できる高親和性のトランスフェリンです。ヒトホロトランスフェリンは、鉄で飽和させてから使用するか、鉄欠乏または鉄不含細胞培養培地に直接添加します。
生物化学的／生理学的作用
生理学的に安定し安全な形態で細胞に鉄を供給します。
調製ノート
60°C(以上)で10時間以上熱処理済みです。
アナリシスノート
ICPによる鉄含量は、ロットごとに報告されています。
アガロースゲル電気泳動によって純度測定
免責事項
研究用途に限ります。本製品は、輸出入活動を含めて、科学的な目的で使用することを意図した場合、フランスにおいて規制されます（フランス公衆衛生法Article L1211-1 paragraph 2）。購入者（すなわちエンドユーザー）は、公衆衛生法Article L1245-5-1 IIの記載に従い、フランス研究省から輸入許可を得る必要があります。本製品を注文することにより、適切な輸入許可を取得していることを確認することになります。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
T0665-BULK:
T0665-50MG:
T0665-1G:
T0665-500MG:
2020154:
T0665-100MG:
T0665-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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プロトコル
Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.
関連コンテンツ
Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.
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What is the recommended storage method for stock solutions of item T0665, holo-Transferrin human?
1 回答-
役に立ちましたか？
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What's the process of saturation? Do the cells express directly holo-Tf with iron? Or apo-Tf reacts with iron salts?
1 回答-
The delivery of iron to cells by transferrins is a receptor-mediated and controlled process. Cells regulate the amount of iron they receive from the extracellular environment by varying transferrin receptor expression. Two iron-loaded transferrin molecules bind to one transferrin receptor. Once bound, they are transferred into acidic endosomes within the cell’s interior. At reduced pH, the iron is released and incorporated into intracellular proteins and the storage molecule ferritin. The apo-transferrin (iron-free) is recycled back to the exterior of the cell and released. Each transferrin receptor cycle can deliver up to four atoms of iron.
役に立ちましたか？
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Holo-Tf is described as "iron saturated", but there is a statement "Under normal circumstances, approximately one-third of transferrin iron-binding pockets are filled." I'm confused if the product I got (T0665) is 100% saturated with iron or not.
1 回答-
This product is a Holo-Transferrin form which is generally considered to be iron-saturated. The specification for iron content of this product is 0.10 - 0.35%. Conversely, Apo-Transferrin is considered to be iron deficient. Apo-transferrin product T5391 has an iron content specification of <0.005%. Each Transferrin has the capacity to bind 2 iron (Fe3+) molecules. In a circulating system, the iron content is transitory and rarely (if ever) 100% saturated. This product is tested for total iron content. The number of binding sites that are filled is not measured. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/520/t0665pis.pdf役に立ちましたか？
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What is the recommended holo-transferrin solvent and how should the dissolved protein be stored?
1 回答-
This product is tested for solubility in water at 50 mg/mL. Rates are expected to be comparable when dissolving in an aqueous buffer or medium. Solutions are stable for 5-10 days at 2-8°C. This product has not been evaluated for long-term solution stability. However, medium supplements containing transferrin are stable when stored in aliquots at -20°C for up to one year. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdf役に立ちましたか？
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What is the carbohydrate content of human transferrin?
1 回答-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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