If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
H3149
ヘパリン ナトリウム塩 from porcine intestinal mucosa
Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
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About This Item
おすすめの製品
由来生物
Porcine intestinal mucosa
品質水準
タイプ
Grade I-A
製品種目
BioReagent
フォーム
powder
比活性
≥180 USP units/mg
テクニック
analytical sample preparation: suitable
cell culture | mammalian: suitable
色
beige
溶解性
H2O: 50 mg/mL
アプリケーション
cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES記法
[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O
InChI
1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-
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詳細
アプリケーション
特徴および利点
- さまざまな研究用途に適した高純度化合物
同等製品
保管分類コード
11 - Combustible Solids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
H3149-50KU:
H3149-10KU:
H3149-VAR-9:
H3149-BULK:
H3149-25KU:
H3149-250KU:
H3149-BULK-9:
H3149-100KU:
H3149-VAR:
H3149-500KU-9:
H3149-500KU:
H3149-1MU:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
この製品を見ている人はこちらもチェック
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
役に立ちましたか？
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
役に立ちましたか？
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H3149-10KU heparin sodium salt is steril??
1 回答-
The product is not sterile. Prepared solutions may be filter sterilized.
役に立ちましたか？
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please, could you explain me if this H3149 is one of 'high molecolar weight heparin'? thanks
1 回答-
This product is a cell culture tested porcine heparin with a molecular weight range of 15-19 kDa. This material is considered a "high molecular weight heparin." According to the literaure, low molecular weight heparins are those with a molecular wieght of less that 8000 Da. See the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141002/役に立ちましたか？
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Can this material be diluted to 10,000 U/mL and stored at 2C to 8C? Do you have stability data at this temperature for lower concentrations?
1 回答-
Yes, this product remains biologically active in dilute solutions. Stock solutions prepared at 50 mg/mL in water are stable for up to 2 years at 2 - 8°C, when filter sterilized. According to literature, heparin diluted in physiological saline at a concentration of 10 to 100 units/mL is stable for at least 35 days when stored at 2 - 8°C. Please see the link below to review the datasheet for an identical product, H3393, which may be applied to this item
H3393 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdf役に立ちましたか？
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Is the heparin sodium salt (H3149, CAS 9041-08-1) unfractionated or fractionated? How does this classification relate to its molecular weight?"
1 回答-
The heparin sodium salt (H3149, CAS 9041-08-1) is unfractionated. This classification is reflected in its molecular weight, which ranges approximately from 9,000 to 21,000, with the majority falling between 17,000-19,000. As confirmed by our product and as indicated in the USP monograph, our heparin is unfractionated. This differs from fractionated Heparin, also known as Low Molecular Weight Heparin, which has a much lower molecular weight. According to the DBR, 3rd ed., p. 342, the molecular weight of our heparin is approximately 16,000-17,000.
役に立ちましたか？
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Can you recommend a suitable heparin product for mouse heart perfusion?
1 回答-
Our available heparin products have not undergone specific testing for mouse heart perfusion. However, you may find H3149 of interest as it has been cited in the following publication for this intended application: "Isolation and Primary Culture of Mouse Aortic Endothelial Cells" published in J Vis Exp. 2016; (118): 52965 (PMID: 28060318).
役に立ちましたか？
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Is it a different substance from heparan sulfate?
1 回答-
Heparin is not equivalent to heparin sulfate. Please see the link below to describe the differences:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/what-is-heparin役に立ちましたか？
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Can I dissolve the heparin in filtered (0.22 um) nuclease free water at a concentration of 50mg/ml? Does it correspond to 5000 IU/ml? Then, I can use 15-30 IU/ ml of blood to avoid its coagulation, is it correct?
1 回答-
As mentioned on the Product Detail Page, this product is soluble at 50 mg/mL in water. The actual activity is reported on the lot-specific Certificate of Analysis. This value should be used in determining the activity of the prepared solution. The activity specification for this product is >/=180 USP units/mg. For example, assuming an activity value of 200 units/mg, a prepared solution of 50 mg/ml will have an activity of 5000 units/ml. Yes, typically, 15 - 30 units/ml is suitable for use as an anticoagulant.
役に立ちましたか？
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Can we store the solution of Heparin sodium from intestinal mucosa of pig in RNase free water at -25 C?
1 回答-
Solutions can be stored at 2-8 °C for up to 2 years, if sterile filtered through a 0.22 micron filter. The freeze/thaw process may cause degradation of the polysaccharide.
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