SRP0415

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• 別名: OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
• CAS番号: 1644286-11-2
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥80% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 59 kDa
• 包装: pkg of 20 μg
• メーカー／製品名: Sigma-Aldrich
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: 1.8 mg/mL
• テクニック: cell based assay: suitable
• 溶解性: water: soluble
• NCBIアクセッション番号: NM_182470
• UniProtアクセッション番号: P14618
• アプリケーション: life science and biopharma
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PKM(5315)

詳細

詳細

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2.[1][2] PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues,[2] retina, adipose tissues, and islets.[1] The PKM1 gene is located on the human chromosome at 15q23.[3]

アプリケーション

PKM1active human has been used in:

• cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.[4]
• oxidative stress experiments on PKM2.[5]
• Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).[6]

生物化学的／生理学的作用

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.[1]

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H360D
• 注意書き: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable