Yes, this material has a fluorescence spectrum similar to DAPI, with excitation at 340-380 nm and emission at 385-470 nm. The images displayed above were acquired using UV filter set (340-380 nm excitation, 40 nm dichroic, 430-nm long pass filter). Please note that Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately.
おすすめの製品
詳細
Filipin III is a polyene macrolide antibiotic and the major component isolated from cultures of S. filipinensis. 1 Due to its ability to fluoresce and bind to cholesterol, Filipin has been widely
used as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for free cholesterol in the study of Type C Niemann-Pick disease. 3 Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. 4 Filipin III was found to trigger signaling responses in tobacco cells, including a NADPH oxidase-dependent production. 5. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
used as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for free cholesterol in the study of Type C Niemann-Pick disease. 3 Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. 4 Filipin III was found to trigger signaling responses in tobacco cells, including a NADPH oxidase-dependent production. 5. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
アプリケーション
Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.
その他情報
- Filipin III solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C avoid freeze thaw cycles.
- Filipin III is used in various concentrations depending on the specific protocol used. Dilute 1mg/ml stock solution with appropriate buffer according to the used protocol.
- Filipin III interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
188.6 °F
引火点(℃)
87 °C
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
消防法
第4類:引火性液体
第三石油類
危険等級III
非水溶性液体
Jan Code
SAE0087-VAR:
SAE0087-BULK:
SAE0087-1ML-PW:
SAE0087-1ML:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
この製品を見ている人はこちらもチェック
Pan Gao et al.
Investigative ophthalmology & visual science, 63(5), 32-32 (2022-05-27)
Bietti crystalline dystrophy (BCD) is a progressive retinal degenerative disease primarily characterized by numerous crystal-like deposits and degeneration of retinal pigment epithelium (RPE) and photoreceptor cells. CYP4V2 (cytochrome P450 family 4 subfamily V member 2) is currently the only disease-causing
Yun-Qi Zhou et al.
Emerging microbes & infections, 11(1), 1135-1144 (2022-03-29)
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants is threatening public health around the world. Endocytosis functions as an important way for viral infection, and SARS-CoV-2 bears no exception. However, the specific endocytic mechanism of
Wen-Bin Zhang et al.
Acta neuropathologica communications, 11(1), 102-102 (2023-06-22)
Accumulating evidences reveal that cellular cholesterol deficiency could trigger the onset of Alzheimer's disease (AD). As a key regulator, 24-dehydrocholesterol reductase (DHCR24) controls cellular cholesterol homeostasis, which was found to be downregulated in AD vulnerable regions and involved in AD-related
Carla E Cadena Del Castillo et al.
Nature communications, 12(1), 4898-4898 (2021-08-14)
Hedgehog (Hh) signaling is essential during development and in organ physiology. In the canonical pathway, Hh binding to Patched (PTCH) relieves the inhibition of Smoothened (SMO). Yet, PTCH may also perform SMO-independent functions. While the PTCH homolog PTC-3 is essential
Filipin, an Antifungal Antibiotic: Isolation and Properties
George B. Whitfield, Thomas D. Brock, Alfred Ammann, David Gottlieb, and Herbert E. Carter
Journal of Chemical and Pharmaceutical Sciences
, 4799-4801 (1955)
-
Would it be possible to image this product using a DAPI filter (Excitation 377 nm, Emission 447 nm)?
1 回答-
役に立ちましたか？
-
-
Any suggestions for addressing white precipitations observed during Filipin staining for endothelial cells, possibly due to DMSO? Also, seeking advice to improve the experiment where most cells were negative for Filipin staining, and only a few were positive while measuring cholesterol content in HUVEC cells.
1 回答-
To address white precipitations observed during Filipin staining for endothelial cells, increasing the amount of DMSO in the final staining solution may be necessary. This can be achieved by diluting the 1 mg/ml Filipin III solution with more DMSO solution to create a 0.5 mg/ml or 0.1 mg/ml concentrated solution prior to staining the samples.
For improving cholesterol content measurement in HUVEC cells, optimize the working concentration of Filipin and conduct a time-course experiment to enhance staining results. Prompt analysis of stained samples can help prevent photobleaching.役に立ちましたか？
-
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）
