Product 1024190250 is intended for In Vitro Diagnostic Use, and the Instructions For Use mention staining cryosections but not cultured cells attached to either a culture plate or a chamber slide. Staining cultured cells would be considered "off label" use and would require validation, especially in a clinical lab setting. If the dry dye is used to prepare Oil Red O staining solution, the process for preparing the staining solution would be the same for both cryosections and cells attached to a cell culture plate or chamber slide.
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品質水準
フォーム
liquid
体外診断薬
for in vitro diagnostic use
テクニック
microbe id | staining: suitable
転移温度
flash point -11 °C
密度
0.838 g/cm3
アプリケーション
clinical testing
diagnostic assay manufacturing
hematology
histology
保管温度
15-25°C
詳細
Oil red O staining solution - for the detection of neutral lipids in cryosections - for microscopy, is ready-to-use staining solution that results in a brilliant, bright red staining of lipds (triglycerides), while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Product number 1.05175). Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues of human origin, in joint pathology or in cytological sample material. The stain is carried out on cryo sections of native tissue.
Approx. 200 stains can be performed with one 250 ml bottle. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Approx. 200 stains can be performed with one 250 ml bottle. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
アナリシスノート
Suitability for microscopy: passes test
Lipids: bright red
Nuclei: blue
Lipids: bright red
Nuclei: blue
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
ターゲットの組織
Central nervous system
補足的ハザード
保管分類コード
3 - Flammable liquids
WGK
WGK 2
引火点(°F)
12.2 °F
引火点(℃)
-11 °C
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
消防法
第4類:引火性液体
第一石油類
危険等級II
水溶性液体
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
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Does Oil Red O staining solution 1024190250 also work for cells in culture?
1 回答-
役に立ちましたか？
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Need to know whether or not the Oil red O staining solution (1024190250) works in mice liver?
1 回答-
Oil Red O is soluble in water and the solubility test is conducted to 1 mg per ml. The actual maximum solubility can be higher, up to 10 mg/ml, although this may. However, the solubility test is specific to water and may differ if used in agar.
役に立ちましたか？
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Is the Propanol in the Oil red O staining solution Halal en Kosher ?
1 回答-
The Oil red O staining solution is prepared with ethanol and acetone. Neither of these components are certified Kosher or Halal. The staining protocol requires 2-propanol. In the product information sheet 1.09634, 2-isopropanol, is suggested. This product is also not certified Halal or Kosher. See the link below to review this protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/144/102419-en.pdfThe food grade 2-propanol products do offer this certification. See the link below for an example: https://www.sigmaaldrich.com/US/en/product/aldrich/w292907
役に立ちましたか？
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Does this work to in mouse liver frozen slices?
1 回答-
Oil Red O lacks the specificity of an antibody. The basic theory of fat or lipid staining is that the dye is dissolved in a solvent with minimal solubility. A working solution is made just prior to use to further decrease the solubility. The dye then moves from the solution with minimal solubility to the solvent where there is better solubility. In this case the dye moves from the solution to the fat or lipid being stained. It matters not whether the tissue is from a human, mouse or other animals.
This information is true to most fat or lipid stains. But with Oil Red O the solution is prepared to a working solution and no further dilution is required.
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