Vacuolar protein sorting-associated protein 4 (Vps4) is a member of the AAA protein family (ATPases associated with diverse cellular activities). Vps4 exists as two orthologs in humans (VPS4A and VPS4B). The gene encoding Vps4A is mapped to chromosome 16q22, while the gene for Vps4B resides on chromosome 18q21-q22.
特異性
Anti-VPS4 recognizes human, mouse, and rat VPS4A and VPS4B.
アプリケーション
Anti-VPS4 antibody has been used in
immunoprecipitation
immunofluorescence Confocal Microscopy
immunohistochemistry
western blotting
生物化学的／生理学的作用
Vacuolar protein sorting-associated protein 4 (VPS4) mediates endosomal membrane protein sorting, retroviral budding and cytokinesis by recognizing membrane-associated ESCRT-III assemblies and catalyzing their disassembly.
物理的形状
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
保管および安定性
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Thoresen SB, et al.
Nature Cell Biology, 16(6), 550-560 (2014)
The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting of alpha-synuclein
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VPS4 is a dynamic component of the centrosome that regulates centrosome localization of $\gamma$-tubulin, centriolar satellite stability and ciliogenesis
