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ligand
glutathione
包装
pack of 100 mL
メーカー/製品名
Cytiva 17-0756-05
Matrix
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
4-13
動作範囲
4-13
キャパシティ
>25 mg binding capacity (horse liver GST/ml medium)
関連するカテゴリー
詳細
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
特徴および利点
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
プロトコル
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
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