In general, MEM typically contains 0.292 g/L L-Glutamine. A 200mM Glutamine solution is equivalent to 29.23 mg/mL. So it needs to be diluted around 100 times.
おすすめの製品
無菌性
sterile-filtered
品質水準
300
400
製品種目
BioXtra
フォーム
solution
濃度
200 mM
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
輸送温度
dry ice
保管温度
−20°C
SMILES記法
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI Key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
遺伝子情報
rat ... Ggt1(116568)
類似した製品をお探しですか？ 訪問 製品比較ガイド
詳細
L-グルタミンは、培地の重要な成分である必須アミノ酸であり、培養中に細胞の主要エネルギー源として機能します。L-グルタミンは、乾燥粉末としても凍結溶液としても非常に安定しています。しかし、液体培地または保存溶液中では、L-グルタミンは比較的急速に分解します。最適な細胞パフォーマンスを得るためには、通常、使用前に L-グルタミンを培地に補給する必要があります。
アプリケーション
L-グルタミンは必須アミノ酸の1つで、重要な培地の構成成分であり、培養細胞の主なエネルギー源となります。細胞の性能を最適にするためには、通常は培地を使用する前にL-グルタミンを補充する必要があります。
調製ノート
細胞培養グレ-ド水で調製されています。
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
アプリケーション
推奨
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
G7513-BULK:
G7513-PH:
G7513-450ML:4548173198279
G7513-20ML:4548173198262
G7513PROC:
G7513-PROC:
G7513-500ML:
G7513-20ML-EW:
G7513-6X100ML:
G7513-100ML:4548173198255
G7513-VAR:
G7513-1L:
この製品を見ている人はこちらもチェック
Nara Shin et al.
Environmental science. Processes & impacts, 21(8), 1342-1352 (2019-05-03)
In this study, we investigated the airborne particles released during paper printing and paper shredding processes in an attempt to characterize and differentiate these particles. Particle characteristics were studied with real time instruments (RTIs) to measure concentrations and with samplers
Zlata Vershinin et al.
The Journal of biological chemistry, 291(13), 6786-6795 (2016-02-05)
Lysine methylation of non-histone proteins has emerged as a key regulator of many cellular functions. Although less studied than other post-translational modifications such as phosphorylation and acetylation, the number of known methylated non-histone proteins is rapidly expanding. We have identified
Boris Redko et al.
Oncotarget, 8(1), 757-768 (2016-10-22)
The newly discovered short (9 amino acid) non-RGD S-S bridged cyclic peptide ALOS-4 (H-cycl(Cys-Ser-Ser-Ala-Gly-Ser-Leu-Phe-Cys)-OH), which binds to integrin αvβ3 is investigated as peptide carrier for targeted drug delivery against human metastatic melanoma. ALOS4 binds specifically the αvβ3 overexpressing human metastatic
Miguel Pérez-Garrastachu et al.
Nucleus (Austin, Tex.), 8(5), 515-533 (2017-07-12)
Nucleoporins are the main components of the nuclear-pore complex (NPC) and were initially considered as mere structural elements embedded in the nuclear envelope, being responsible for nucleocytoplasmic transport. Nevertheless, several recent scientific reports have revealed that some nucleoporins participate in
Ofir Cohn et al.
Scientific reports, 6, 37115-37115 (2016-11-16)
SETD3 is a member of the protein lysine methyltransferase (PKMT) family, which catalyzes the addition of methyl group to lysine residues. Accumulating data suggest that PKMTs are involved in the regulation of a broad spectrum of biological processes by targeting
資料
Importance and uses of glutamine in hybridoma and mammalian cell culture
Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.
プロトコル
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
Chromatograms
application for HPLCapplication for HPLC-
How much of Product G7513 should be used in cell culture media?
1 回答-
役に立ちましたか？
-
-
Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 回答-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
役に立ちましたか？
-
-
Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 回答-
L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
役に立ちましたか？
-
-
What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
-
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）
