F1757

フマラーゼ ブタ心臓由来

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

• 別名: L-リンゴ酸ヒドロリアーゼ, フマル酸ヒドラターゼ
• CAS番号: 9032-88-6
• Enzyme Commission number: 4.2.1.2 (BRENDA, IUBMB)
• EC Number: 232-887-7
• MDL番号: MFCD00131111
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• フォーム: ammonium sulfate suspension
• 比活性: ≥300 units/mg protein (biuret)
• その他の活性: malic dehydrogenase ≤0.1%
• 保管温度: 2-8°C

詳細

詳細

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.[1]

Fumarase is mapped to human chromosome 1q43.[2] Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.[3]

アプリケーション

Fumarase from porcine heart has been used:

• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay[4]
• for immobilized enzyme based stability assay[5]
• in fumarate hydratase assays in human lung carcinoma cells (A549)[6]

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.[7]

生物化学的／生理学的作用

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Fumarase is essential for regulation of cellular metabolism.[8] Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation.[9] Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.[2][10]

単位の定義

1ユニットは、pH 7.6、25°C、1分間で、1.0 μmolのL-リンゴ酸をフマル酸へと変換する酵素量です。

物理的形状

0.014 M 2-メルカプトエタノ-ル, 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄(pH 7.5)を含有する溶液で調製した懸濁液

調製ノート

Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977)に記載の方法に従って単離。アフィニティ-精製済み。

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable