The [R-H-K-K(Ac)-AFC] substrate is not specific to just the HDAC3. It is recommended to purify the HDAC3 enzyme if necessary.
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使用法
100 assays in 96 well plates
NCBIアクセッション番号
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
human ... HDAC3(8841)
mouse ... HDAC3(15183)
詳細
Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.
With Sigma′s HDAC3 Activity Assay Kit, HDAC3 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC3 Substrate [R-H-K-K(Ac)-AFC]. This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC3 activity. The kit provides a rapid, simple, sensitive and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC3, and from native, recombinant, or genetically modified HDAC3.
With Sigma′s HDAC3 Activity Assay Kit, HDAC3 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC3 Substrate [R-H-K-K(Ac)-AFC]. This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC3 activity. The kit provides a rapid, simple, sensitive and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC3, and from native, recombinant, or genetically modified HDAC3.
特徴および利点
- Simple, sensitive, and reliable assay
- Simple procedure; takes ~60 min
- Utilizes fluorometric methods
- Sample type: cell and tissue lysates, plasma and serum, other biological fluids
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Convenient 96-well microplate format
- Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples
関連製品
製品番号
詳細
価格
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
188.6 °F - closed cup
引火点(℃)
87 °C - closed cup
適用法令
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