This kit is NOT suitable for flow cytometry. Please see our Duolink® flowPLA Mouse/Rabbit Starter Kits for flow cytometry: DUO94102 (Green), DUO94104 (Far Red), & DUO94105 (Violet).
製品DUO92101は現在お客様の国では販売されていません。 製品に関するお問い合わせはこちら（テクニカルサービス）
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製品種目
Duolink®
品質水準
テクニック
proximity ligation assay: suitable
蛍光検出
λex 594 nm; λem 624 nm (Texas Red®; Zeiss Filter set 31)
適合性
suitable for fluorescence
保管温度
−20°C
関連するカテゴリー
アプリケーション
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
The Duolink® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
特徴および利点
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
法的情報
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
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製品番号
詳細
SDS
- DUO92002Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)SDS
- DUO92004Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)SDS
- DUO92008Duolink® In Situ Detection Reagents RedSDS
- DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS
- DUO82040Duolink® In Situ Mounting Medium with DAPISDS
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
保管分類コード
8A - Combustible corrosive hazardous materials
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
キットコンポーネントの情報を参照してください
PRTR
キットコンポーネントの情報を参照してください
消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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労働安全衛生法名称等を通知すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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最新バージョンのいずれかを選択してください：
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Tan Z, et al.
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New protein?protein interactions of mitochondrial connexin 43 in mouse heart.
Denuc A, et al.
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Missense mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson's disease (PD). However, a potential role of wild-type LRRK2 in idiopathic PD (iPD) remains unclear. Here, we developed proximity ligation assays to assess Ser1292 phosphorylation of LRRK2 and, separately
資料
Duolink™近接ライゲーションアッセイ（PLA）テクノロジーは、神経細胞の相互作用を研究するために使用でき、神経科学の研究を促進します。
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
細胞および組織中のタンパク質の免疫蛍光検出プロトコル
近接ライゲーションアッセイ技術の仕組みと、EGFによるEGFR-HER2二量体化のin situ検出を確認できるタンパク質間相互作用コントロールキットの仕組みについてご紹介します
プロトコル
This page details the Duolink® In Situ Short Protocol for fluorescence detection
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
このページでは、蛍光顕微鏡観察のためのDuolink™ In Situの簡易プロトコルを紹介しています。
関連コンテンツ
タンパク質－RNA、タンパク質－DNA、タンパク質－タンパク質相互作用の検討および関連するアプリケーションのためのタンパク質－核酸相互作用試薬およびリソース。
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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Hello, Is this kit also suitable for flow cytometry-based analysis of the data?
1 回答-
役に立ちましたか？
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Can the Duolink kits be used with a Mouse IgM antibody?
1 回答-
Duolink kits may be compatible with a Mouse IgM antibody, provided that the antibody contains the light chain. The Anti-Mouse MINUS probe specifically interacts with mouse IgG (H+L) and the light chains associated with IgM. Therefore, if the Mouse IgM antibody includes the light chain, it should be suitable for use with the Duolink kits.
役に立ちましたか？
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How many reactions can be done with this kit?
1 回答-
The Duolink™ starter kit contains all the components necessary to analyze up to 30 reactions in one single order. Please see the link below to review the full protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-fluorescence-user-manual#DuolinkPLAFluorescenceProtocol役に立ちましたか？
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I want to study the interaction of two cell surface proteins using PLA kit . My primaries are mouse and rabbit. Does the kit have the two secondary and also all the components that i would require to carry out the assay. Can you advice?
1 回答-
The Duolink® In Situ Red Starter Kit Mouse/Rabbit - DUO92101 contains the Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, affinity purified Donkey anti-Rabbit IgG, and Duolink® In Situ PLA® Probe Anti-Mouse MINUS, affinity purified Donkey anti-Mouse IgG (H+L) secondary antibodies and everything needed to conduct the PLA assay.
役に立ちましたか？
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What is the expiry date of the kit contents?
1 回答-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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