A2237

アルカリホスファターゼ エビ由来

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

• 別名: オルトリン酸-モノエステルホスホヒドロラーゼ(アルカリ性至適)
• CAS番号: 9001-78-9
• Enzyme Commission number: 3.1.3.1 (BRENDA, IUBMB)
• MDL番号: MFCD00131849
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in proprietary host
• 品質水準: 200
• フォーム: buffered aqueous glycerol solution
• 濃度: ≥900 DEA units/mL
• その他の活性: RNAse, DNAse, none detected
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

アプリケーション

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice.[1] It also may be used in pasteurization process for milks used in dairy products.[2]

生物化学的／生理学的作用

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

単位の定義

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

物理的形状

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

アナリシスノート

Protein determined by biuret

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable