21819

Carbol-Fuchsin solution according to Kinyoun

for microscopy

• 別名: Kinyoun’s carbol-Fuchsin
• MDL番号: MFCD00143924
• UNSPSCコード: 12171500
• PubChem Substance ID: 329752209
• NACRES: MA.02

特性

• グレード: for microscopy
• 品質水準: 100
• 形状: liquid
• 組成: fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)
• 不純物: phenol
• 色: red to very dark red
• 密度: 0.99 g/mL at 20 °C
• &ε; （減衰係数）: 77 at 546 nm in 50% ethanol
• アプリケーション: diagnostic assay manufacturing; hematology; histology
• 保管温度: room temp
• SMILES記法: NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]
• InChI: 1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3
• InChI Key: IMOKRVRFBCWWGH-UHFFFAOYSA-N

詳細

詳細

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

アプリケーション

• Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
• It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
• It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

原理

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

その他情報

Stain used for the demonstration of tuberculosis bacteria

安全性情報

• ピクトグラム: GHS02,GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226,H302,H314,H341,H351,H412
• 注意書き: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 3
• 引火点(°F): 109.9 °F - closed cup
• 引火点(℃): 43.3 °C - closed cup