おすすめの製品
品質水準
体外診断薬
for in vitro diagnostic use
アプリケーション
clinical testing
diagnostic assay manufacturing
hematology
histology
保管温度
15-25°C
適合性
(Acid-fast bacteria)
詳細
The AFB-Color modified - Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method, is used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin, for example smears of enriched bacterial cultures.
AFB-Color modified staining set uses the classical Ziehl-Neelsen hot staining procedure and can be used for staining on the staining rack. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. This AFB-Color modified staining kit uses methylene blue as counterstain.
Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material.
The package is sufficient for approx. 250 applications. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
AFB-Color modified staining set uses the classical Ziehl-Neelsen hot staining procedure and can be used for staining on the staining rack. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. This AFB-Color modified staining kit uses methylene blue as counterstain.
Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material.
The package is sufficient for approx. 250 applications. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
アプリケーション
Used to detect acid-fast bacteria" (AFB hot staining)
アナリシスノート
Suitability for microscopypasses test acid-fast bacteria red Background blue
法的情報
SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Danger
危険有害性の分類
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B
保管分類コード
3 - Flammable liquids
WGK
WGK 3
引火点(°F)
69.8 °F
引火点(℃)
21 °C
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
キットコンポーネントの情報を参照してください
PRTR
キットコンポーネントの情報を参照してください
消防法
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を表示すべき危険物及び有害物
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
キットコンポーネントの情報を参照してください
Jan Code
キットコンポーネントの情報を参照してください
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）